Methods Article
Efficient SARS-CoV-2 Quantitative Reverse Transcriptase PCR Saliva Diagnostic Strategy utilizing Open-Source Pipetting Robots
4.3K Views
•
11:11 min
•
February 11th, 2022
DOI :
February 11th, 2022
•Rozdziały w tym wideo
0:04
Introduction
0:56
Sample Intake, Heat Treatment, and Sample Assignment
1:52
Operating Sample Loading Robots
4:09
Manual Sample Loading
4:52
Addition of Controls to Testing Plates
5:54
Performing RT-qPCR and Determining Plate Validity
7:00
Interpreting Sample Results
8:08
Results: RT-qPCR Detection of N1 (SARS-CoV-2) Synthetic RNA and P1 (Hs_RPP30) Synthetic DNA, Comparison Between Manual and Automated Saliva Transfer N1 Ct Values, and Evaluation of Heat Treatment Methods for Viscosity Reduction in Saliva
10:26
Conclusion
Powiązane Filmy
Rapid Screening of HIV Reverse Transcriptase and Integrase Inhibitors
17.7K Views
Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization
5.9K Views
Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection
4.2K Views
A Rapid, Multiplex Dual Reporter IgG and IgM SARS-CoV-2 Neutralization Assay for a Multiplexed Bead-Based Flow Analysis System
4.8K Views
Detection of SARS-CoV-2 Receptor-Binding Domain Antibody using a HiBiT-Based Bioreporter
3.0K Views
Live Imaging and Quantification of Viral Infection in K18 hACE2 Transgenic Mice Using Reporter-Expressing Recombinant SARS-CoV-2
2.6K Views
Application of Ha-CoV-2 Pseudovirus for Rapid Quantification of SARS-CoV-2 Variants and Neutralizing Antibodies
1.2K Views
Pseudotyped Viruses As a Molecular Tool to Monitor Humoral Immune Responses Against SARS-CoV-2 Via Neutralization Assay
1.5K Views
Profiling of Surface Protein Epitopes on Viral Particles by Multiplex Dual-Reporter Strategy
581 Views
Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone