Efficient SARS-CoV-2 Quantitative Reverse Transcriptase PCR Saliva Diagnostic Strategy utilizing Open-Source Pipetting Robots
4.4K Views
•
11:11 min
•
February 11th, 2022
DOI :
February 11th, 2022
•Chapters in this video
0:04
Introduction
0:56
Sample Intake, Heat Treatment, and Sample Assignment
1:52
Operating Sample Loading Robots
4:09
Manual Sample Loading
4:52
Addition of Controls to Testing Plates
5:54
Performing RT-qPCR and Determining Plate Validity
7:00
Interpreting Sample Results
8:08
Results: RT-qPCR Detection of N1 (SARS-CoV-2) Synthetic RNA and P1 (Hs_RPP30) Synthetic DNA, Comparison Between Manual and Automated Saliva Transfer N1 Ct Values, and Evaluation of Heat Treatment Methods for Viscosity Reduction in Saliva
10:26
Conclusion
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