Efficient SARS-CoV-2 Quantitative Reverse Transcriptase PCR Saliva Diagnostic Strategy utilizing Open-Source Pipetting Robots
4.6K Views
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11:11 min
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February 11th, 2022
DOI :
February 11th, 2022
•Rachel E. Ham*1, Austin R. Smothers*1,2, Kylie L. King1, Justin M. Napolitano1, Theodore J. Swann3, Lesslie G. Pekarek4, Mark A. Blenner*1,5,6, Delphine Dean*1,2
1Center for Innovative Medical Devices and Sensors (REDDI Lab), Clemson University, 2Department of Bioengineering, Clemson University, 3Swann Medicine, 4Student Health Services, Clemson University, 5Department of Chemical & Biomolecular Engineering, Clemson University, 6Department of Chemical & Biomolecular Engineering, University of Delaware
Chapters in this video
0:04
Introduction
0:56
Sample Intake, Heat Treatment, and Sample Assignment
1:52
Operating Sample Loading Robots
4:09
Manual Sample Loading
4:52
Addition of Controls to Testing Plates
5:54
Performing RT-qPCR and Determining Plate Validity
7:00
Interpreting Sample Results
8:08
Results: RT-qPCR Detection of N1 (SARS-CoV-2) Synthetic RNA and P1 (Hs_RPP30) Synthetic DNA, Comparison Between Manual and Automated Saliva Transfer N1 Ct Values, and Evaluation of Heat Treatment Methods for Viscosity Reduction in Saliva
10:26
Conclusion
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