Genome-wide Profiling of Transcription Factor-DNA Binding Interactions in Candida albicans: A Comprehensive CUT&RUN Method and Data Analysis Workflow
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07:48 min
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April 1st, 2022
DOI :
April 1st, 2022
•Mohammad N. Qasim*1,2, Ashley Valle Arevalo*1,2, Akshay D. Paropkari*1,2, Craig L. Ennis1,2,4, Suzanne S. Sindi3, Clarissa J. Nobile1,4, Aaron D. Hernday1,4
1Department of Molecular and Cell Biology, University of California, Merced, 2Quantitative and Systems Biology Graduate Program, University of California, Merced, 3Department of Applied Mathematics, University of California, Merced, 4Health Sciences Research Institute, University of California, Merced
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Rozdziały w tym wideo
0:04
Introduction
0:49
Isolation of Nuclei and Primary Antibody Binding
2:40
Binding of pAG-MNase to Antibody
3:36
Library Preparation for Sequencing
5:22
CUT&RUN Sequence Analysis
5:54
Results: Quality Control Steps and Evaluation of Ndt80 and Efg1 Enriched Peaks Using CUT&RUN Protocol
7:22
Conclusion
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