A Competent Hepatocyte Model Examining Hepatitis B Virus Entry through Sodium Taurocholate Cotransporting Polypeptide as a Therapeutic Target
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2.1K Views
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11:34 min
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May 10th, 2022
DOI :
May 10th, 2022
•Khanit Sa-ngiamsuntorn1, Piyanoot Thongsri2, Yongyut Pewkliang2, Suparerk Borwornpinyo3,4, Adisak Wongkajornsilp5
1Department of Biochemistry, Faculty of Pharmacy, Mahidol University, 2Program in Translational Medicine, Faculty of Medicine Ramathibodi Hospital, Mahidol University, 3Excellent Center for Drug Discovery, Faculty of Science, Mahidol University, 4Department of Biotechnology, Faculty of Science, Mahidol University, 5Department of Pharmacology, Faculty of Medicine Siriraj Hospital, Mahidol University
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Rozdziały w tym wideo
0:05
Introduction
0:50
Measuring the Sodium Taurocholate Co-Transporting Polypeptide (NTCP) Using Flow Cytometry
4:30
Anti-HBV (Hepatitis B Virus) Entry Assay
6:13
HBV (Hepatitis B Virus) -Cell Binding Assay
6:50
Taurocholic Acid Uptake Assay
7:34
Determination of Protein-Ligand Interactions Using Isothermal Titration Calorimetry
9:14
Results: Screening of Anti-Hepatitis B Virus Using Isothermal Titration Calorimetry
10:36
Conclusion
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