A Semi-Automated and Reproducible Biological-Based Method to Quantify Calcium Deposition In Vitro

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1.9K Views

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11:30 min

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June 2nd, 2022

DOI :

10.3791/64029-v

June 2nd, 2022

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Armand M. G. Jaminon*¹, Nikolas Rapp*¹, Asim C. Akbulut¹, Robert Dzhanaev², Chris P. Reutelingsperger¹, Willi Jahnen-Dechent², Leon J. Schurgers¹^,³

¹Department of Biochemistry, Cardiovascular Research Institute Maastricht (CARIM), Maastricht University, ²Helmholtz Institute for Biomedical Engineering, Biointerface Group, RWTH Aachen University, ³Institute of Experimental Medicine and Systems Biology, RWTH Aachen University

Podsumowanie

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Calcium Deposition

Vascular Calcification

Human Vascular Smooth Muscle Cells

Fluorescent Detection

Calcification Propensity

Cell Culture

Trypsin Reaction

Calcification Medium

Light Microscope Imaging

Rozdziały w tym wideo

0:05

Introduction

0:36

Cell Seeding, Maintenance, and Calcification Induction

2:13

Calcification Detection via Imaging

7:51

Data Analysis

10:13

Results: A Semi-Automated Method for In Vitro Calcification Determination

11:05

Conclusion

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