Contact Hypersensitivity as a Murine Model of Allergic Contact Dermatitis

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2.4K Views

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08:25 min

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September 26th, 2022

DOI :

10.3791/64329-v

September 26th, 2022

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Magdalena Zemelka-Wiacek¹, Monika Majewska-Szczepanik², Pawel Gajdanowicz¹, Marian Szczepanik³

¹Department of Clinical Immunology, Wroclaw Medical University, ²Department of Medical Physiology, Chair of Biomedical Sciences, Institute of Physiotherapy, Faculty of Health Sciences, Jagiellonian University Medical College, ³Chair of Biomedical Sciences, Faculty of Health Sciences, Jagiellonian University Medical College

Podsumowanie

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Contact Hypersensitivity

Murine Model

Allergic Contact Dermatitis

Ear Edema

T cell Immune Responses

Myeloperoxidase Assay

Experimental Methods

Immunological Response

Rozdziały w tym wideo

0:04

Introduction

0:59

Marking Mice for Identification, and Induction of Contact Hypersensitivity (CHS)

2:19

Ear Biopsies for Ear Weight and Myeloperoxidase (MPO) Assay

3:48

Vascular Permeability Test

4:43

Serum Collection and Anti-TNP Immunoglobulin (IgG1) Antibody Measurement

5:16

Adoptive Transfer of the CHS-Effector Cells

6:41

Results: Study of the Contact Hypersensitivity Reaction in Mice

7:42

Conclusion

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