The question we intend to answer with this study is to evaluate nociception in laboratory animals experimentally-infected with Trypanosoma evansi, as observed in previous studies by our group. In relation to Trypanosoma evansi, the most recent developments are regarding prevalence studies in various locations around the globe, followed by the search for specific biomarkers for differential diagnosis. Some experimental challenges are mainly related to animal welfare.
All the care related to the three Rs must be reviewed and encouraged. We believe that an article with video instruction, as we show in this study, is a good incentive to discuss reduction in experimental models. Our experiments enable the necessity of analgesics during Trypanosoma evansi infection, since this infection was able to diminish the nociception threshold in our model.
We can now ask ourselves how the inflammation, allodynia and pain caused by Trypanosoma evansi infection affects the disease's progression. We also need to understand how it molecularly impacts the hosts'clinical sites and behavior, as well as the parasites'behavior inside infected organism.
