In this report, we present a stepwise approach to stimulate single fiber EMG in rodents. Additionally, in a rat model of brocorion-induced neurovascular junction transmission failure, we demonstrate the all or non appearance of single fiber action potentials and provide representative examples of increased jitter and blocking. Single fiber EMG is a well established clinical diagnostic test, but its application in preclinical studies is limited.
Our protocol bridges this gap by clearly outlining the procedural steps for qualifying jitter and blocking to characterize rodent models of aging in a neuromuscular disease. Our lab focuses on translational neuromuscular physiology in the context of health, aging, and disease. By leveraging techniques that can be applied both in preclinical and clinical studies, our findings can be more readily translatable to improve human health.
