experimental-viral-infection-adult-mosquitoes-oral-feeding

Experimental Viral Infection in Adult Mosquitoes by Oral Feeding and Microinjection

Mosquito-borne viruses (MBVs), which are infectious pathogens to vertebrates, are spread by many mosquito species, posing a severe threat to public ...

Chinese Academy of Sciences

University of Chinese Academy of Sciences

Orbiviruses are arboviruses with 10 double-stranded linear RNA segments, and some have been identified as pathogens of dramatic epizootics in both wild and domestic ruminants. Tibet orbivirus (TIBOV) is a new orbivirus isolated from hematophagous insects in recent decades, and, currently, most of the strains have been isolated from insects in PR China, except for two from Japan. In this study, we isolated a novel reassortment TIBOV strain, YN15-283-01, from  spp. To identify and understand more characteristics of YN15-283-01, electrophoresis profiles of the viral genome, electron microscopic observations, plaque assays, growth curves in various cell lines, and bioinformatic analysis were conducted. The results indicated that YN15-283-01 replicated efficiently in mosquito cells, rodent cells and several primate cells. Furthermore, the maximum likelihood phylogenetic trees and simplot analysis of the 10 segments indicated that YN15-283-01 is a natural reassortment isolate that had emerged mainly from XZ0906 and SX-2017a.

Christabel Ochieng

Key Laboratory of Special Pathogens and Biosafety,
Wuhan Institute of Virology,
Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology

Chinese Academy of Sciences

Experimental Viral Infection in Adult Mosquitoes by Oral Feeding and Microinjection

Christabel Ochieng

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Chinese Academy of Sciences

Experimental Viral Infection in Adult Mosquitoes by Oral Feeding and Microinjection

Key Laboratory of Special Pathogens and Biosafety,
Wuhan Institute of Virology,
Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology