polarization-sensitive-two-photon-microscopy-for-label-free-amyloid

Polarization-Sensitive Two-Photon Microscopy for a Label-Free Amyloid Structural Characterization

Compared to its one-photon counterpart, two-photon excitation is beneficial for bioimaging experiments because of its lower phototoxicity, deeper tissue ...

Wrocław University of Science and Technology

This review provides a comprehensive description of nonlinear optical (NLO) properties of gold nanoparticles, which can be used in biological applications. The main focus is placed on two-photon absorption (2PA) and two-photon excited photoluminescence (2PEL) - the processes crucial for multiphoton microscopy, which allows deeper imaging of the material and causes less damage to the biological samples in comparison to conventional (one-photon) microscopy. We present the basics of 2PA measurement techniques and a summary of recent achievements in the understanding of multiphoton excitation and the resulting photoluminescence in gold nanoparticles, both plasmonic ones and small nanoclusters with molecule-like properties. The examples of 2PA applications in bioimaging are also presented, with a comment on future challenges and applications.

Two-photon absorption and photoluminescence of colloidal gold nanoparticles and nanoclusters.

Amyloids are broadly investigated protein misfolding products with characteristic β-sheet assemblies that have an important role in neurodegenerative diseases (e.g., Alzheimer's disease). While they are usually visualized by staining with Thioflavin-T, Congo Red, or other fluorescent markers, it still arouses a controversy over possible staining molecule influence on the amyloid structure or aggregation process. In this work we present, for the first time, the polarization analysis of two-photon excited autofluorescence of amyloids and confirm that polarization dependence of the observed emission can be correlated with the orientation of fibrils. We show the potential of two-photon excited autofluorescence for resolution of molecular organization of fibrils within amyloid superstructures. This label-free method is compatible with two-photon imaging already applied in investigation of neurodegeneration model in mice.

Two-Photon Excited Polarization-Dependent Autofluorescence of Amyloids as a Label-Free Method of Fibril Organization Imaging.

Chiral nanomaterials attract broad attention, as they offer new possibilities of modulation of optical properties and dissymmetry factors outperforming organic materials. Among various nanoparticles, plasmonic bipyramids present numerous advantages as building blocks of chiral nanomaterials (well-defined modulation of optical properties with the morphology of nanoparticles, narrow optical resonances, and high size and shape uniformity of synthesized particles). We study different possible orientations of gold bipyramids with respect to each other in dimers obtained by wet chemistry methods. For circularly polarized incident light we evaluate linear optical cross sections and plasmonic local field enhancement using COMSOL Multiphysics. We observe coupling of the nanoparticles' local fields and thus changes in extinction spectra, which modulate chiroptical properties of dimers. To assess the chirality of various arrangements, we note differences in cross sections for left- and right-handed polarized light which we further evaluate as the dissymmetry -factor. Our results provide BPs configurations with dissymmetry factor as high as -0.3.

Circular Dichroism of Gold Bipyramid Dimers.

Protein misfolding products - amyloids - tend to form distinct fibrillar structures of the characteristic fold for a given neurodegenerative disease or pathology. Moreover, amyloids (also in the intermediate or distorted state) can act as secondary nuclei for  fibrillation. Such secondary nucleation amplifies plaque development correlated with various diseases. Therefore, a versatile and non-destructive method of detection and differentiation between distinct fibrillar structures is of great importance. Amyloids exhibit unique optical properties,  green-blue autofluorescence, which can also be induced by two-photon excitation. Herein, we use this label-free technique to resolve local fibrillar ordering in amyloid superstructures - spherulites. With polarization-dependent two-photon excited amyloid autofluorescence, we resolved fibrillar orientation in the spherulite corona and discussed the presence of amorphous aggregates, distorted fibrils or amyloid intermediate species within the spherulite core. Our polarization sensitive two-photon microscopy investigations are supported by TEM imaging and provide a promising tool for the detection and differentiation between well-developed amyloid fibrils and amorphous/distorted structures present at different stages of the formation of amyloid superstructures and plaques.

Amyloid fibrils in superstructures - local ordering revealed by polarization analysis of two-photon excited autofluorescence.

Noble metal nanoclusters allow for the atomically-precise control of their composition. However, to create nanoclusters with pre-defined optical properties, comprehensive description of their structure-property relation is required. Here, we report the gold atom doping impact on one-photon and two-photon absorption (TPA) and luminescence properties of ligated silver nanoclusters via combined experimental studies and time-dependent density functional theory simulations (TD-DFT). We synthesized a series of Ag Au (DMBT) nanoclusters where x=0, 1 and 5-10. For Ag Au (DMBT) we demonstrate that the presence of the central Au dopant strongly influences linear and non-linear optical properties, increasing photoluminescence quantum yield and two-photon brightness, with respect to undoped silver nanoclusters. With improved TPA and luminescence, atomically-precise AuAg alloys presented in our work can serve as robust luminescent probes e.g. for bioimaging in the second biological window.

Gold-Doping Effect on Two-Photon Absorption and Luminescence of Atomically Precise Silver Ligated Nanoclusters.

The distinct polarity of biomolecule surfaces plays a pivotal role in their biochemistry and functions as it is involved in numerous processes, such as folding, aggregation, or denaturation. Therefore, there is a need to image both hydrophilic and hydrophobic bio-interfaces with markers of distinct responses to hydrophobic and hydrophilic environments. In this work, we present a synthesis, characterization, and application of ultrasmall gold nanoclusters capped with a 12-crown-4 ligand. The nanoclusters present an amphiphilic character and can be successfully transferred between aqueous and organic solvents and have their physicochemical integrity retained. They can serve as probes for multimodal bioimaging with light (as they emit near-infrared luminescence) and electron microscopy (due to the high electron density of gold). In this work, we used protein superstructures, namely, amyloid spherulites, as a hydrophobic surface model and individual amyloid fibrils with a mixed hydrophobicity profile. Our nanoclusters spontaneously stained densely packed amyloid spherulites as observed under fluorescence microscopy, which is limited for hydrophilic markers. Moreover, our clusters revealed structural features of individual amyloid fibrils at a nanoscale as observed under a transmission electron microscope. We show the potential of crown ether-capped gold nanoclusters in multimodal structural characterization of bio-interfaces where the amphiphilic character of the supramolecular ligand is required.

Crown Ether-Capped Gold Nanoclusters as a Multimodal Platform for Bioimaging.

The COVID-19 pandemic has stimulated the scientific world to intensify virus-related studies aimed at the development of quick and safe ways of detecting viruses in the human body, studying the virus-antibody and virus-cell interactions, and designing nanocarriers for targeted antiviral therapies. However, research on dangerous viruses can only be performed in certified laboratories that follow strict safety procedures. Thus, developing deactivated virus constructs or safe-to-use virus-like objects, which imitate real viruses and allow performing virus-related studies in any research laboratory, constitutes an important scientific challenge. Such species, called virus-like particles (VLPs), contain instead of capsids with viral DNA/RNA empty or synthetic cores with real virus proteins attached to them. We have developed a method for the preparation of VLPs imitating the virus responsible for the COVID-19 disease: the SARS-CoV-2. The particles have Au cores surrounded by "coronas" of S1 domains of the virus's spike protein. Importantly, they are safe to use and specifically interact with SARS-CoV-2 antibodies. Moreover, Au cores exhibit localized surface plasmon resonance (LSPR), which makes the synthesized VLPs suitable for biosensing applications. During the studies, the effect allowed us to visualize the interaction between the VLPs and the antibodies and identify the characteristic vibrational signals. What is more, additional functionalization of the particles with a fluorescent label revealed their potential in studying specific virus-related interactions. Notably, the universal character of the developed synthesis method makes it potentially applicable for fabricating VLPs imitating other life-threatening viruses.

Patryk Obstarczyk

Institute of Advanced Materials

Wrocław University of Science and Technology

Polarization-Sensitive Two-Photon Microscopy for a Label-Free Amyloid Structural Characterization

Patryk Obstarczyk

.css-o250i3{font-size:18px;font-family:Open Sans,sans-serif;line-height:21.6px;}Institute of Advanced Materials

Wrocław University of Science and Technology

Polarization-Sensitive Two-Photon Microscopy for a Label-Free Amyloid Structural Characterization

Institute of Advanced Materials