Sergei Zolotukhin

Efficient photoreceptor-targeted gene expression in vivo by recombinant adeno-associated virus.

Transduction of human and mouse pancreatic islet cells using a bicistronic recombinant adeno-associated viral vector.

Streamlined large-scale production of recombinant adeno-associated virus (rAAV) vectors.

Production and purification of serotype 1, 2, and 5 recombinant adeno-associated viral vectors.

Identification of amino acid residues in the capsid proteins of adeno-associated virus type 2 that contribute to heparan sulfate proteoglycan binding.

Sustained peripheral expression of transgene adiponectin offsets the development of diet-induced obesity in rats.

Adeno-associated virus type 2 VP2 capsid protein is nonessential and can tolerate large peptide insertions at its N terminus.

Recombinant AAV viral vectors pseudotyped with viral capsids from serotypes 1, 2, and 5 display differential efficiency and cell tropism after delivery to different regions of the central nervous system.

Efficient transduction of vascular endothelial cells with recombinant adeno-associated virus serotype 1 and 5 vectors.

Gene therapy for obesity.

Successful production of pseudotyped rAAV vectors using a modified baculovirus expression system.

Production, purification, crystallization and preliminary X-ray analysis of adeno-associated virus serotype 8.

Production, purification, crystallization and preliminary X-ray structural studies of adeno-associated virus serotype 5.

Production, purification and preliminary X-ray crystallographic studies of adeno-associated virus serotype 1.

A dual role of EGFR protein tyrosine kinase signaling in ubiquitination of AAV2 capsids and viral second-strand DNA synthesis.

Ectopic expression of Wnt10b decreases adiposity and improves glucose homeostasis in obese rats.

Structure of adeno-associated virus serotype 8, a gene therapy vector.

Production, purification and preliminary X-ray crystallographic studies of adeno-associated virus serotype 7.

Synergy between leptin therapy and a seemingly negligible amount of voluntary wheel running prevents progression of dietary obesity in leptin-resistant rats.

Optimization of recombinant adeno-associated viral vectors for human beta-globin gene transfer and transgene expression.

Next generation of adeno-associated virus 2 vectors: point mutations in tyrosines lead to high-efficiency transduction at lower doses.

Tyrosine-phosphorylation of AAV2 vectors and its consequences on viral intracellular trafficking and transgene expression.

High-efficiency transduction of the mouse retina by tyrosine-mutant AAV serotype vectors.

An inducible system for highly efficient production of recombinant adeno-associated virus (rAAV) vectors in insect Sf9 cells.

Production, purification and preliminary X-ray crystallographic studies of adeno-associated virus serotype 9.

An animal model of PDH deficiency using AAV8-siRNA vector-mediated knockdown of pyruvate dehydrogenase E1α.

High-efficiency transduction and correction of murine hemophilia B using AAV2 vectors devoid of multiple surface-exposed tyrosines.

Neutralizing antibodies against AAV serotypes 1, 2, 6, and 9 in sera of commonly used animal models.

Salivary PYY: a putative bypass to satiety.

Structural insight into the unique properties of adeno-associated virus serotype 9.

High-efficiency transduction of human monocyte-derived dendritic cells by capsid-modified recombinant AAV2 vectors.

Distribution of Y-receptors in murine lingual epithelia.

Development of Novel Recombinant AAV Vectors and Strategies for the Potential Gene Therapy of Hemophilia.

Structural insights into adeno-associated virus serotype 5.

Modulation of taste responsiveness by the satiation hormone peptide YY.

Salivary peptide tyrosine-tyrosine 3-36 modulates ingestive behavior without inducing taste aversion.

OneBac: platform for scalable and high-titer production of adeno-associated virus serotype 1-12 vectors for gene therapy.

Vector design Tour de Force: integrating combinatorial and rational approaches to derive novel adeno-associated virus variants.

Identification of low-expressing transcripts of the NPY receptors' family in the murine lingual epithelia.

A simplified purification protocol for recombinant adeno-associated virus vectors.

OneBac 2.0: Sf9 Cell Lines for Production of AAV5 Vectors with Enhanced Infectivity and Minimal Encapsidation of Foreign DNA.

E Pluribus Unum: 50 Years of Research, Millions of Viruses, and One Goal--Tailored Acceleration of AAV Evolution.

Altering Tropism of rAAV by Directed Evolution.

Adeno-Associated Virus Type 2 and Hepatocellular Carcinoma?

Snord116 is critical in the regulation of food intake and body weight.

High-accuracy biodistribution analysis of adeno-associated virus variants by double barcode sequencing.

Prader-Willi Critical Region, a Non-Translated, Imprinted Central Regulator of Bone Mass: Possible Role in Skeletal Abnormalities in Prader-Willi Syndrome.

Limited ATF4 Expression in Degenerating Retinas with Ongoing ER Stress Promotes Photoreceptor Survival in a Mouse Model of Autosomal Dominant Retinitis Pigmentosa.

OneBac 2.0: Sf9 Cell Lines for Production of AAV1, AAV2, and AAV8 Vectors with Minimal Encapsidation of Foreign DNA.

Evaluation of engineered AAV capsids for hepatic factor IX gene transfer in murine and canine models.

An Immune-Competent Murine Model to Study Elimination of AAV-Transduced Hepatocytes by Capsid-Specific CD8 T Cells.

Direct Head-to-Head Evaluation of Recombinant Adeno-associated Viral Vectors Manufactured in Human versus Insect Cells.

Helper-free Production of Laboratory Grade AAV and Purification by Iodixanol Density Gradient Centrifugation.

Achieving High-Yield Production of Functional AAV5 Gene Delivery Vectors via Fedbatch in an Insect Cell-One Baculovirus System.

Taste Receptor Cells in Mice Express Receptors for the Hormone Adiponectin.

Removal of Endotoxin from rAAV Samples Using a Simple Detergent-Based Protocol.

Engineering and Selection of a Novel AAV3B Variant with High Hepatocyte Tropism and Reduced Seroreactivity.

Applying machine learning to predict viral assembly for adeno-associated virus capsid libraries.

The CRTC1-MAML2 fusion is the major oncogenic driver in mucoepidermoid carcinoma.

Mixture interactions at mammalian olfactory receptors are dependent on the cellular environment.

A comprehensive study of a 29-capsid AAV library in a non-human primate central nervous system.

Y1 receptors modulate taste-related behavioral responsiveness in male mice to prototypical gustatory stimuli.

Scratching the surface of RGD-directed AAV capsid engineering.

AAV capsid design: A Goldilocks challenge.

Identification of Novel Retinal Pericyte-Targeting rAAV Vectors Through Directed Evolution.

Characterization of a Bioengineered AAV3B Capsid Variant with Enhanced Hepatocyte Tropism and Immune Evasion.

Intravitreal injection of a rationally designed AAV capsid library in non-human primate identifies variants with enhanced retinal transduction and neutralizing antibody evasion.

Exploring the Comprehensive Kozak Sequence Landscape for AAV Production in Sf9 System.

Rational Design of AAV-rh74, AAV3B, and AAV8 with Limited Liver Targeting.