Atsushi Kuno

RNA aptamers targeted to domain II of hepatitis C virus IRES that bind to its apical loop region.

In vitro selection of RNA aptamers that bind to domain II of HCV IRES.

Structure-inhibition analysis of RNA aptamers that bind to HCV IRES.

A hepatitis C virus (HCV) internal ribosome entry site (IRES) domain III-IV-targeted aptamer inhibits translation by binding to an apical loop of domain IIId.

Evanescent-field fluorescence-assisted lectin microarray: a new strategy for glycan profiling.

Overexpression, purification and crystallization of tyrosyl-tRNA synthetase from the hyperthermophilic archaeon Aeropyrum pernix K1.

Characterization of an exo-beta-1,3-galactanase from Clostridium thermocellum.

Evidence that Agaricus bisporus agglutinin (ABA) has dual sugar-binding specificity.

The family 42 carbohydrate-binding module of family 54 alpha-L-arabinofuranosidase specifically binds the arabinofuranose side chain of hemicellulose.

A combined strategy for glycan profiling: a model study with pyridylaminated oligosaccharides.

Inhibition of tumor cell-induced platelet aggregation using a novel anti-podoplanin antibody reacting with its platelet-aggregation-stimulating domain.

Functional glycosylation of human podoplanin: glycan structure of platelet aggregation-inducing factor.

Tailoring a novel sialic acid-binding lectin from a ricin-B chain-like galactose-binding protein by natural evolution-mimicry.

A novel strategy for mammalian cell surface glycome profiling using lectin microarray.

Polylactosamine on glycoproteins influences basal levels of lymphocyte and macrophage activation.

Engineering of mucin-type human glycoproteins in yeast cells.

Development of an all-in-one technology for glycan profiling targeting formalin-embedded tissue sections.

Optimization of evanescent-field fluorescence-assisted lectin microarray for high-sensitivity detection of monovalent oligosaccharides and glycoproteins.

Focused differential glycan analysis with the platform antibody-assisted lectin profiling for glycan-related biomarker verification.

Strategy for glycoproteomics: identification of glyco-alteration using multiple glycan profiling tools.

Engineering a versatile tandem repeat-type alpha2-6sialic acid-binding lectin.

Mechanism by which the lectin actinohivin blocks HIV infection of target cells.

A strategy for discovery of cancer glyco-biomarkers in serum using newly developed technologies for glycoproteomics.

Wisteria floribunda agglutinin-positive mucin 1 is a sensitive biliary marker for human cholangiocarcinoma.

Beta3GnT2 (B3GNT2), a major polylactosamine synthase: analysis of B3GNT2-deficient mice.

Differential glycan profiling by lectin microarray targeting tissue specimens.

A versatile technology for cellular glycomics using lectin microarray.

Multilectin assay for detecting fibrosis-specific glyco-alteration by means of lectin microarray.

Lectin-based structural glycomics: a practical approach to complex glycans.

LecT-Hepa: A triplex lectin-antibody sandwich immunoassay for estimating the progression dynamics of liver fibrosis assisted by a bedside clinical chemistry analyzer and an automated pretreatment machine.

High Throughput ELISAs to Measure a Unique Glycan on Transferrin in Cerebrospinal Fluid: A Possible Extension toward Alzheimer's Disease Biomarker Development.

Directed evolution of lectins with sugar-binding specificity for 6-sulfo-galactose.

A serum "sweet-doughnut" protein facilitates fibrosis evaluation and therapy assessment in patients with viral hepatitis.

Glycoproteomic discovery of serological biomarker candidates for HCV/HBV infection-associated liver fibrosis and hepatocellular carcinoma.

Glycoproteomics-based cancer marker discovery adopting dual enrichment with Wisteria floribunda agglutinin for high specific glyco-diagnosis of cholangiocarcinoma.

Reconstruction of a robust glycodiagnostic agent supported by multiple lectin-assisted glycan profiling.

Multilectin-assisted fractionation for improved single-dot tissue glycome profiling in clinical glycoproteomics.

Application of a glycoproteomics-based biomarker development method: alteration in glycan structure on colony stimulating factor 1 receptor as a possible glycobiomarker candidate for evaluation of liver cirrhosis.

Novel glycobiomarker for ovarian cancer that detects clear cell carcinoma.

IgA nephropathy caused by unusual polymerization of IgA1 with aberrant N-glycosylation in a patient with monoclonal immunoglobulin deposition disease.

Differential glycan analysis of an endogenous glycoprotein: toward clinical implementation--from sample pretreatment to data standardization.

Glycoproteomics approach for identifying Glycobiomarker candidate molecules for tissue type classification of non-small cell lung carcinoma.

Wisteria floribunda agglutinin positive human Mac-2-binding protein as a predictor of hepatocellular carcinoma development in chronic hepatitis C patients.

LecT-Hepa facilitates estimating treatment outcome during interferon therapy in chronic hepatitis C patients.

Clinicopathological characteristics and diagnostic performance of Wisteria floribunda agglutinin positive Mac-2-binding protein as a preoperative serum marker of liver fibrosis in hepatocellular carcinoma.

Development and Applications of the Lectin Microarray.

Lectin microarray technology identifies specific lectins related to lymph node metastasis of advanced gastric cancer.

Serum Wisteria Floribunda Agglutinin-Positive Mac-2 Binding Protein Values Predict the Development of Hepatocellular Carcinoma among Patients with Chronic Hepatitis C after Sustained Virological Response.

Lectin Microarray-Based Sero-Biomarker Verification Targeting Aberrant O-Linked Glycosylation on Mucin 1.

Verification of WFA-Sialylated MUC1 as a Sensitive Biliary Biomarker for Human Biliary Tract Cancer.

A novel glycobiomarker, Wisteria floribunda agglutinin macrophage colony-stimulating factor receptor, for predicting carcinogenesis of liver cirrhosis.

Influenza A Virus-Induced Expression of a GalNAc Transferase, GALNT3, via MicroRNAs Is Required for Enhanced Viral Replication.

NMR analysis on the sialic acid-binding mechanism of an R-type lectin mutant by natural evolution-mimicry.

Alteration of matrix metalloproteinase-3 O-glycan structure as a biomarker for disease activity of rheumatoid arthritis.

Serum WFA -M2BP levels for evaluation of early stages of liver fibrosis in patients with chronic hepatitis B virus infection.

Wisteria floribunda agglutinin-sialylated mucin core polypeptide 1 is a sensitive biomarker for biliary tract carcinoma and intrahepatic cholangiocarcinoma: a multicenter study.

Hepatic stellate cells secreting WFA -M2BP: Its role in biological interactions with Kupffer cells.

A standardized method for lectin microarray-based tissue glycome mapping.

Serum Wisteria Floribunda Agglutinin-Positive Sialylated Mucin 1 as a Marker of Progenitor/Biliary Features in Hepatocellular Carcinoma.

Assessment of tumor characteristics based on glycoform analysis of membrane-tethered MUC1.

Mac-2 binding protein glycan isomer (M2BPGi) is a new serum biomarker for assessing liver fibrosis: more than a biomarker of liver fibrosis.

Highly Sensitive Glycan Profiling of Hepatitis B Viral Particles and a Simple Method for Dane Particle Enrichment.

Current Technologies for Complex Glycoproteomics and Their Applications to Biology/Disease-Driven Glycoproteomics.

Response to the letter by Dr. Naoya Yamada, and Dr. Koichi Mizuta regarding our manuscript: "Mac-2 binding protein glycan isomer (M2BPGi) is a new serum biomarker for assessing liver fibrosis: more than a biomarker of liver fibrosis".

Lectin microarray analyses reveal host cell-specific glycan profiles of the hemagglutinins of influenza A viruses.

An Improved Method for Cell Type-Selective Glycomic Analysis of Tissue Sections Assisted by Fluorescence Laser Microdissection.

Wisteria floribunda agglutinin staining for the quantitative assessment of cardiac fibrogenic activity in a mouse model of dilated cardiomyopathy.

Lectin Bead Array in a Single Tip Facilitates Fully Automatic Glycoprotein Profiling.

LM-GlycomeAtlas Ver. 1.0: A Novel Visualization Tool for Lectin Microarray-Based Glycomic Profiles of Mouse Tissue Sections.

Glycomic Signatures of Plasma IgG Improve Preoperative Prediction of the Invasiveness of Small Lung Nodules.

GlycoBIST: A System for Automatic Glycan Profiling of a Target Protein Using Milli-Bead Array in a Tip.

Discovery of Pancreatic Ductal Adenocarcinoma-Related Aberrant Glycosylations: A Multilateral Approach of Lectin Microarray-Based Tissue Glycomic Profiling With Public Transcriptomic Datasets.

Comparative Glycomic Analysis of Exosome Subpopulations Derived from Pancreatic Cancer Cell Lines.

Multi-serum glycobiomarkers improves the diagnosis and prognostic prediction of cholangiocarcinoma.

Screening siRNAs against host glycosylation pathways to develop novel antiviral agents against hepatitis B virus.

C-Terminally tagged NA in replication-competent influenza A viruses reveals differences in glycan profiles between NA and HA.

-Glycan-Altered Extracellular Vesicles: A Specific Serum Marker Elevated in Pancreatic Cancer.

LM-GlycomeAtlas Ver. 2.0: An Integrated Visualization for Lectin Microarray-based Mouse Tissue Glycome Mapping Data with Lectin Histochemistry.

N-glycan structures of Wisteria floribunda agglutinin-positive Mac2 binding protein in the serum of patients with liver fibrosis†.

Enhanced Azidolysis by the Formation of Stable Ser-His Catalytic Dyad in a Glycoside Hydrolase Family 10 Xylanase Mutant.

O-glycosylated HBsAg peptide can induce specific antibody neutralizing HBV infection.

Tissue Glycome Mapping: Lectin Microarray-Based Differential Glycomic Analysis of Formalin-Fixed Paraffin-Embedded Tissue Sections.

ST6GAL1 inhibits metastasis of hepatocellular carcinoma via modulating sialylation of MCAM on cell surface.

Reduction in disialyl-T antigen levels in mice deficient for both St6galnac3 and St6galnac4 results in blood filling of lymph nodes.

An improved evanescent fluorescence scanner suitable for high-resolution glycome mapping of formalin-fixed paraffin-embedded tissue sections.

Chondroitin sulfate modification of CSPG4 regulates the maintenance and differentiation of glioma-initiating cells via integrin-associated signaling.

Differential Glycoform Analysis of MUC1 Derived from Biological Specimens Using an Antibody-Overlay Lectin Microarray.

Role of Wisteria floribunda agglutinin binding glycans in carcinogenesis and metastasis of cholangiocarcinoma.

Glycosylation of recombinant adeno-associated virus serotype 6.