depletion-of-ribosomal-rna-for-mosquito-gut-metagenomic-rna-seq

Depletion of Ribosomal RNA for Mosquito Gut Metagenomic RNA-seq

The mosquito gut accommodates dynamic microbial communities across different stages of the insect's life cycle. Characterization of the genetic capacity ...

New Mexico State University

The mosquito gut represents an ecosystem that accommodates a complex, intimately associated microbiome. It is increasingly clear that the gut microbiome influences a wide variety of host traits, such as fitness and immunity. Understanding the microbial community structure and its dynamics across mosquito life is a prerequisite for comprehending the symbiotic relationship between the mosquito and its gut microbial residents. Here we characterized gut bacterial communities across larvae, pupae and adults of Anopheles gambiae reared in semi-natural habitats in Kenya by pyrosequencing bacterial 16S rRNA fragments. Immatures and adults showed distinctive gut community structures. Photosynthetic Cyanobacteria were predominant in the larval and pupal guts while Proteobacteria and Bacteroidetes dominated the adult guts, with core taxa of Enterobacteriaceae and Flavobacteriaceae. At the adult stage, diet regime (sugar meal and blood meal) significantly affects the microbial structure. Intriguingly, blood meals drastically reduced the community diversity and favored enteric bacteria. Comparative genomic analysis revealed that the enriched enteric bacteria possess large genetic redox capacity of coping with oxidative and nitrosative stresses that are associated with the catabolism of blood meal, suggesting a beneficial role in maintaining gut redox homeostasis. Interestingly, gut community structure was similar in the adult stage between the field and laboratory mosquitoes, indicating that mosquito gut is a selective eco-environment for its microbiome. This comprehensive gut metatgenomic profile suggests a concerted symbiotic genetic association between gut inhabitants and host.

Dynamic gut microbiome across life history of the malaria mosquito Anopheles gambiae in Kenya.

L&#39;intestin du moustique repr&eacute;sente un &eacute;cosyst&egrave;me qui abrite un microbiome complex, intimement li&eacute;. Il est de plus en plus &eacute;vident que le microbiome intestinale influence une grande vari&eacute;t&eacute; de traits de l&#39;h&ocirc;te, tels que la remise en forme et l&#39;immunit&eacute;. Comprendre la structure de la communaut&eacute; microbienne et sa dynamique &agrave; travers la vie de moustique est une condition pr&eacute;alable pour comprendre la relation symbiotique entre le moustique et ses r&eacute;sidents microbienne de l&#39;intestin. Ici nous avons caract&eacute;ris&eacute; l&#39;intestin des communaut&eacute;s bact&eacute;riennes dans les larves, les nymphes et les adultes d&#39;Anopheles gambiae &eacute;lev&eacute;es dans des habitats semi-naturels au Kenya par pyrosequencing bact&eacute;rienne 16 s rRNA fragments. Immatures et les adultes ont montr&eacute; des structures communautaires gut distinctif. Cyanobact&eacute;ries photosynth&eacute;tiques sont pr&eacute;dominants dans les larves et les nymphes tripes tout en prot&eacute;obact&eacute;ries et Bacteroidetes domin&eacute; les tripes adultes, avec les taxons de base des Enterobacteriaceae et Flavobacteriaceae. Au stade adulte, r&eacute;gime r&eacute;gime alimentaire (repas de sucre et la farine de sang) affecte de mani&egrave;re significative la structure microbienne. Curieusement, repas de sang a consid&eacute;rablement r&eacute;duit la diversit&eacute; des communaut&eacute;s et des ent&eacute;robact&eacute;ries favoris&eacute;es. Analyse g&eacute;nomique comparative a r&eacute;v&eacute;l&eacute; que les bact&eacute;ries ent&eacute;riques enrichis poss&egrave;dent capacit&eacute; grand r&eacute;dox g&eacute;n&eacute;tique de coping with oxydatif et contraintes nitrosative qui sont associ&eacute;s avec le catabolisme de la farine de sang, ce qui sugg&egrave;re un r&ocirc;le b&eacute;n&eacute;fique dans le maintien de l&#39;hom&eacute;ostasie redox gut. Fait int&eacute;ressant, structure tube digestif de la Communaut&eacute; &eacute;tait similaire chez les adultes entre les moustiques de terrain et en laboratoire, indiquant que l&#39;intestin du moustique est eco-milieu s&eacute;lectif pour ses microbiome. Le profil de ce tube digestif complet metatgenomic sugg&egrave;re une association g&eacute;n&eacute;tique symbiotique concert&eacute;e entre les habitants de l&#39;intestin et de la foule.

Microbiome gut dynamique &agrave; travers l&#39;histoire de vie de la paludisme moustique Anopheles gambiae au Kenya.

L&#39;intestino della zanzara rappresenta un ecosistema che ospita un complesso, intimamente associato microbioma. &Egrave; sempre pi&ugrave; chiaro che il microbioma intestinale influenza un&#39;ampia variet&agrave; di tratti di host, come il fitness e l&#39;immunit&agrave;. Comprendere la struttura della comunit&agrave; microbica e la sua dinamica attraverso la vita di zanzara &egrave; un requisito indispensabile per comprendere il rapporto simbiotico tra la zanzara e suoi residenti microbica intestinale. Qui abbiamo caratterizzato gut comunit&agrave; batteriche attraverso le larve, pupe e adulti di Anopheles gambiae allevate in habitat seminaturali in Kenya da pyrosequencing batterici 16S rRNA frammenti. Immaturi e adulti ha mostrato strutture comunitarie caratteristico budello. Cianobatteri fotosintetici erano predominanti nella pancia mentre Proteobacteria e paludose e Bacteroidetes dominato il coraggio degli adulti, con taxa di nucleo di Enterobacteriaceae e Flavobacteriaceae. Allo stadio adulto, regime di dieta (farina zucchero e farine di sangue) incide in misura significativa la struttura microbica. Curiosamente, i pasti di sangue ridotto drasticamente la diversit&agrave; della Comunit&agrave; e batteri enterici favorite. Analisi genomica comparativa ha rivelato che i batteri enterici arricchiti possiedono capacit&agrave; grande genetica redox di fronteggiare ossidativo e A&beta; sottolinea che &egrave; associati con il catabolismo del pasto di sangue, suggerendo un ruolo benefico nel mantenimento dell&#39;omeostasi redox di budello. &Egrave; interessante notare, gut comunit&agrave; struttura era simile nella fase adulta tra le zanzare campo e in laboratorio, che indica che l&#39;intestino della zanzara &egrave; un eco-ambiente selettivo per il microbioma. Questo profilo metatgenomic gut completa suggerisce una concertata associazione simbiotica genetica tra gli abitanti di budello e host.

Dinamica intestinale microbioma in tutta la storia della vita della malaria zanzara Anopheles gambiae in Kenya.

El intestino del mosquito representa un ecosistema que alberga un microbioma complejo, &iacute;ntimamente asociado. Es cada vez m&aacute;s claro que el microbioma intestinal influye en una amplia variedad de rasgos de host, como fitness y la inmunidad. Entender la estructura de la comunidad microbiana y su din&aacute;mica a trav&eacute;s de la vida del mosquito es un prerrequisito para comprender la relaci&oacute;n simbi&oacute;tica entre el mosquito y sus residentes microbiana del intestino. Aqu&iacute; caracterizamos gut comunidades bacterianas de larvas, pupas y adultos de Anopheles gambiae criadas en h&aacute;bitats seminaturales en Kenia por Pirosecuenciaci&oacute;n bacteriano 16S rRNA fragmentos. Inmaduros y adultos demostraron las estructuras comunitarias de tripa distintivo. Cianobacterias fotosint&eacute;ticas eran predominantes en las entra&ntilde;as de larvas y pupas mientras proteobacterias y Bacteroidetes hab&iacute;a dominado las agallas para adultos, con taxa de n&uacute;cleo de Enterobacteriaceae y Flavobacteriaceae. En la etapa adulta, r&eacute;gimen de dieta (comida de az&uacute;car y harina de sangre) afecta significativamente la estructura microbiana. Curiosamente, comidas de sangre reduce dr&aacute;sticamente la diversidad de la comunidad y bacterias ent&eacute;ricas favorecidas. An&aacute;lisis gen&oacute;mico comparativo revelaron que las bacterias ent&eacute;ricas enriquecidas poseen capacidad redox gen&eacute;tica grande de sobrellevar oxidativo y nitrosativo subraya que se asocian con el catabolismo de la harina de sangre, lo que sugiere un papel beneficioso en el mantenimiento de la homeostasis redox de tripa. Curiosamente, estructura de la comunidad de tripa era similar en la etapa adulta entre los mosquitos de campo y laboratorio, indicando que el intestino del mosquito es un selectivo respetuosos del medio ambiente para su microbioma. Este perfil de metatgenomic de tripa integral sugiere una asociaci&oacute;n gen&eacute;tica simbi&oacute;tica concertada entre habitantes de la tripa y el receptor.

Microbioma intestinal din&aacute;mico a trav&eacute;s de la historia de la vida de la malaria mosquito Anopheles gambiae en Kenia.

A Pseudomonas sp. bacterium was isolated from the midguts of Anopheles gambiae mosquitoes. Here we present the annotated Pseudomonas sp. draft genome sequence as a contribution to the efforts of characterization of the mosquito gut microbiome.

Draft genome sequence of Pseudomonas sp. strain Ag1, isolated from the midgut of the malaria mosquito Anopheles gambiae.

Bact&eacute;rie Pseudomonas sp., a &eacute;t&eacute; isol&eacute;e de l&#39;intestin moyen des moustiques Anopheles gambiae. Nous pr&eacute;sentons ici annot&eacute; Pseudomonas sp. projet s&eacute;quen&ccedil;age du g&eacute;nome comme une contribution aux efforts de caract&eacute;risation de la moustique gut microbiome.

Le projet de s&eacute;quen&ccedil;age du g&eacute;nome de la souche Pseudomonas sp Ag1, isol&eacute;es de l&#39;intestin moyen de la paludisme moustique Anopheles gambiae.

Un batterio di Pseudomonas sp., &egrave; stato isolato dall&#39;intestino medio delle zanzare di Anopheles gambiae. Qui vi presentiamo l&#39;annotata Pseudomonas sp. bozza sequenza del genoma come un contributo agli sforzi di caratterizzazione del microbioma di intestino zanzara.

Progetto di sequenza del genoma del ceppo di Pseudomonas sp. Ag1, isolato dall&#39;intestino della malaria zanzara Anopheles gambiae.

An isolate of Enterobacter sp. was obtained from the microbial community within the gut of the Anopheles gambiae mosquito, a major malaria vector in Africa. This genome was sequenced and annotated. The genome sequences will facilitate subsequent efforts to characterize the mosquito gut microbiome.

Draft genome sequences of Enterobacter sp. isolate Ag1 from the midgut of the malaria mosquito Anopheles gambiae.

Un isolat de Enterobacter SP a &eacute;t&eacute; obtenu de la communaut&eacute; microbienne dans l&#39;intestin de l&#39;anoph&egrave;le gambiae, un vecteur majeur contre le paludisme en Afrique. Ce g&eacute;nome a &eacute;t&eacute; s&eacute;quenc&eacute; et annot&eacute;. Les s&eacute;quences du g&eacute;nome facilitera les efforts subs&eacute;quents pour caract&eacute;riser le microbiome de tube digestif du moustique.

Projet des s&eacute;quences de g&eacute;nomes d&#39;Enterobacter SP. isolent Ag1 de l&#39;intestin moyen de la paludisme moustique Anopheles gambiae.

Isolate di Enterobacter SP &egrave; stata ottenuta da parte della comunit&agrave; microbica all&#39;interno del budello della zanzara Anopheles gambiae, un vettore importante malaria in Africa. Questo genoma &egrave; stato sequenziato e annotato. Le sequenze di genoma faciliter&agrave; i successivi sforzi di caratterizzare il microbioma intestinale zanzara.

Phanidhar Kukutla

Department of Biology

New Mexico State University

Depletion of Ribosomal RNA for Mosquito Gut Metagenomic RNA-seq

Phanidhar Kukutla

.css-o250i3{font-size:18px;font-family:Open Sans,sans-serif;line-height:21.6px;}Department of Biology

New Mexico State University

Depletion of Ribosomal RNA for Mosquito Gut Metagenomic RNA-seq

Department of Biology