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Agency for Science, Technology and Research (A STAR)

1 ARTICLES PUBLISHED IN JoVE

Biochemistry

Human Mesenchymal Stem Cell Processing for Clinical Applications Using a Closed Semi-Automated Workflow

Alan Tin Lun Lam¹, Premkumar Jayaraman², Abigail Becker³, Ryan Lim², Kim Leng Teo¹, Jacqueline Ng², Steve Oh¹

¹Bioprocessing Technology Institute (BTI), Agency for Science, Technology and Research (A STAR), ²Thermo Fisher Scientific, ³Thermo Fisher Scientific

Here, we present a protocol to harvest adherent cells from multi-layered flasks in a closed semi-automated manner using a counterflow centrifugation system. This protocol can be applied for harvesting both adherent and suspension cells from other cell expansion platforms with few modifications to the existing steps.