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University of Science and Technology Chittagong (USTC)

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Quantifying Myeloperoxidase-DNA and Neutrophil Elastase-DNA Complexes from Neutrophil Extracellular Traps by Using a Modified Sandwich ELISA

Md. Monirul Islam¹, Umme Salma², Takayuki Irahara², Eizo Watanabe², Naoshi Takeyama²

¹Department of Biochemistry and Biotechnology, University of Science and Technology Chittagong (USTC), ²Department of Emergency and Critical Care Medicine, Aichi Medical University

We present a protocol for a modified sandwich enzyme-linked immunosorbent assay technique to quantitatively measure two components of neutrophil extracellular trap remnants, myeloperoxidase conjugated-DNA and neutrophil elastase conjugated-DNA complexes,derived from activated neutrophils.