Semi-Automated Phenotypic Analysis of Functional 3D Spheroid Cell CulturesStephanie Stransky 1, Dejauwne Young 1, Karoline Mikkelsen 2,3, Annemette Præstegaard Thulesen 2, Helle Sedighi Frandsen 3, Simone Sidoli 1
1Department of Biochemistry, Albert Einstein College of Medicine, 2Department of Biochemistry and Molecular Biology, University of Southern Denmark, 3CelVivo ApS
We present a protocol for growing high-reproducible spheroids and their phenotypic characterization using image capture and proteomics.