Isolation and Culture of Primary Cochlear Hair Cells from Neonatal MiceKun-Yi Wu *1, Bo-Tao Wang *2, Yun-Jia He 2, Jing-Yi Xie 2, Zi-Chen Chen 2, Ying Gao 2
1Department of Core Research Laboratory, The Second Affiliated Hospital of Xi’an Jiaotong University, 2Department of Otolaryngology, The Second Affiliated Hospital of Xi’an Jiaotong University
Herein, we present a detailed protocol for isolating and culturing primary cochlear hair cells from mice. Initially, the organ of Corti was dissected from neonatal (aged 3-5 days) murine cochleae under a microscope. Subsequently, cells were enzymatically digested into a single-cell suspension and identified using immunofluorescence after several days in culture.
