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7 ARTICLES PUBLISHED IN JoVE

Biology

Chromatin Immunoprecipitation (ChIP) using Drosophila tissue

¹Department of Biology, Johns Hopkins University

Recently high-throughput sequencing technology has greatly increased sensitivity of Chromatin Immunoprecipitation (ChIP) experiment and prompted its application using purified cells or dissected tissue. Here we delineate a method to use ChIP technique with Drosophila tissue, which can address the endogenous chromatin state in a well-characterized biological system.

Bioengineering

Engineering Platform and Experimental Protocol for Design and Evaluation of a Neurally-controlled Powered Transfemoral Prosthesis

Fan Zhang¹, Ming Liu¹, Stephen Harper^2,3, Michael Lee³, He Huang¹

¹Joint Department of Biomedical Engineering, North Carolina State University & University of North Carolina at Chapel Hill, ²Department of Physical Medicine and Rehabilitation, University of North Carolina School of Medicine, ³Atlantic Prosthetics & Orthotics, LLC

Neural-machine interfaces (NMI) have been developed to identify the user's locomotion mode. These NMIs are potentially useful for neural control of powered artificial legs, but have not been fully demonstrated. This paper presented (1) our designed engineering platform for easy implementation and development of neural control for powered lower limb prostheses and (2) an experimental setup and protocol in a laboratory environment to evaluate neurally-controlled artificial legs on patients with lower limb amputations safely and efficiently.

Medicine

Photoselective Vaporesection of the Prostate via an End-firing Lithium Triborate Crystal Laser

Xin Wang ¹, Yao-Guang Zhang¹, Sheng-Cai Zhu¹, Ben Wan¹, Ming Liu¹, Jian-Ye Wang¹

¹Department of Urology, Beijing Hospital, National Center of Gerontology

Here we present a protocol to perform Photoselective Vaporesection of the Prostate (PVRP) for benign prostatic hyperplasia (BPH) treatment.

Medicine

Enucleation of the Prostate for the Treatment of Benign Prostatic Hyperplasia Using a 980 nm Diode Laser

Yaqun Zhang^*1, Chunlong Fu^*1,2, Jinfu Wang¹, Pengjie Wu¹, Xin Chen¹, Huimin Hou¹, Xinda Song¹, Wanli Cheng¹, Jianye Wang¹, Ming Liu^1,2

¹Urology, Beijing Hospital, National Center of Gerontology, Beijing Hospital, ²Graduate School of Peking Union Medical College, Beijing Hospital

Here, we present a protocol for modified 980 nm diode laser enucleation to treat large volume benign prostatic hyperplasia.

Medicine

Technical Modification of the Terminal Ureter During Total Transperitoneal Laparoscopic Nephroureterectomy for Upper Urinary Tract Urothelial Carcinoma

Chunlong Fu^*1,2, Yaqun Zhang^*1, Xin Wang¹, Shengcai Zhu¹, Huimin Hou¹, Shengjie Liu¹, Cheng Pang¹, Xinda Song¹, Jia Chen¹, Jianye Wang¹, Ming Liu^1,2

¹(Urology) Beijing Hospital, National Center of Gerontology, The Joint Unit of the Chinese Academy of Medical Sciences, ²Graduate School of Peking Union Medical College, Beijing Hospital

Here, we present a protocol to increase the surgical field of view and reduce the difficulty of total transperitoneal laparoscopic nephroureterectomy surgery by precutting the umbilical ligament before treating the terminal ureter.

Biology

Super-Resolution Live Cell Imaging of Subcellular Structures

Rajesh Ranjan¹, Xin Chen¹

¹Department of Biology, The Johns Hopkins University

Presented here is a protocol for super-resolution live-cell imaging in intact tissue. We have standardized the conditions for imaging a highly sensitive adult stem cell population in its native tissue environment. This technique involves balancing temporal and spatial resolution to allow for the direct observation of biological phenomena in live tissue.

Neuroscience

Characteristics of Pain Changes in Rats with Nerve Injury Within 24 hours After One-Time Tuina Intervention

Zhenjie Yang¹, Sachula Wu¹, Tianyuan Yu¹, Jinping Chen¹, Runlong Zhang¹, Hourong Wang², Yingqi Zhang¹

¹School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, ²Beijing Hospital

We present a protocol using the tuina manipulation simulator to perform the "Three-Manipulation and Three-Acupoint" tuina therapy for minor chronic constriction injury rats and evaluate the effective analgesic time points of tuina within 24 h by testing pain changes through behavioral analysis and changes in inflammatory factor expression using enzyme-linked immunosorbent assay.