Hallym University
2 ARTICLES PUBLISHED IN JoVE
Medicine
A Rat Carotid Balloon Injury Model to Test Anti-vascular Remodeling Therapeutics
Natalia Petrasheskaya 1, Hyun-Jin Tae 1,2, Ismayil Ahmet 1, Mark I. Talan 1, Edward G. Lakatta 1, Li Lin 1
1Laboratory of Cardiovascular Sciences, National Institute on Aging, 2Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University
The rat carotid balloon injury model described below allows researchers to evaluate drugs or therapeutics that negate injury-induced arterial hyperplasia. Detailed pre-surgical preparation, surgical procedure, and post-surgical cares of the animal are described.
Genetics
An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells
Hyun Ah Seo 1, Cho Yean Hwang 1, Sokviseth Moeng 1, Jong Kook Park 1
1Department of Biomedical Science and Research Institute for Bioscience & Biotechnology, Hallym University
This protocol uses a probe-based real-time polymerase chain reaction (PCR), a sulforhodamine B (SRB) assay, 3’ untranslated regions (3’ UTR) cloning, and a luciferase assay to verify the target genes of a miRNA of interest and to understand the functions of miRNAs.
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