Accedi

Hallym University

2 ARTICLES PUBLISHED IN JoVE

Medicine

A Rat Carotid Balloon Injury Model to Test Anti-vascular Remodeling Therapeutics

Natalia Petrasheskaya¹, Hyun-Jin Tae^1,2, Ismayil Ahmet¹, Mark I. Talan¹, Edward G. Lakatta¹, Li Lin¹

¹Laboratory of Cardiovascular Sciences, National Institute on Aging, ²Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University

The rat carotid balloon injury model described below allows researchers to evaluate drugs or therapeutics that negate injury-induced arterial hyperplasia. Detailed pre-surgical preparation, surgical procedure, and post-surgical cares of the animal are described.

Genetics

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells

Hyun Ah Seo¹, Cho Yean Hwang¹, Sokviseth Moeng¹, Jong Kook Park¹

¹Department of Biomedical Science and Research Institute for Bioscience & Biotechnology, Hallym University

This protocol uses a probe-based real-time polymerase chain reaction (PCR), a sulforhodamine B (SRB) assay, 3’ untranslated regions (3’ UTR) cloning, and a luciferase assay to verify the target genes of a miRNA of interest and to understand the functions of miRNAs.