Covalent Fragment Screening Using the Quantitative Irreversible Tethering AssayCharles P. Brown 1,2, Alan Armstrong 1, David J. Mann 2
1Department of Chemistry, Imperial College London, Molecular Sciences Research Hub, 2Department of Life Sciences, Imperial College London, South Kensington Campus
The quantitative irreversible tethering (qIT) assay is a fluorescence-based method of identifying covalent fragments that selectively engage with a target protein. Here, a detailed protocol is outlined to describe the qIT assay, with example results included.
