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State University of New York at Buffalo

4 ARTICLES PUBLISHED IN JoVE

Biology

The Logic, Experimental Steps, and Potential of Heterologous Natural Product Biosynthesis Featuring the Complex Antibiotic Erythromycin A Produced Through E. coli

Ming Jiang¹, Haoran Zhang², Blaine A. Pfeifer¹

¹Chemical and Biological Engineering Department, State University of New York at Buffalo, ²Chemical Engineering Department, Massachusetts Institute of Technology

The heterologous biosynthesis of erythromycin A through E. coli includes the following experimental steps: 1) genetic transfer; 2) heterologous reconstitution; and 3) product analysis. Each step will be explained in the context of the motivation, potential, and challenges in producing therapeutic natural products using E. coli as a surrogate host.

Behavior

Using Unidirectional Rotations to Improve Vestibular System Asymmetry in Patients with Vestibular Dysfunction

Nayer Rassaian¹, Navid G. Sadeghi¹, Bardia Sabetazad², Kathleen M. McNerney³, Robert F. Burkard⁴, Soroush G. Sadeghi⁵

¹Department of Physiology, Shahid Beheshti University of Medical Sciences and Health Services, ²Audiology and Dizziness Center, Dey General Hospital, ³Department of Speech-Language Pathology, SUNY Buffalo State, ⁴Department of Rehabilitation Science, School of Public Health and Health Professions, State University of New York at Buffalo, ⁵Center for Hearing and Deafness, Department of Communicative Disorders and Sciences, State University of New York at Buffalo

A new rehabilitation method is presented for rebalancing the vestibular system in patients with asymmetric responses, which consists of unidirectional rotations toward the weaker side. By directly modifying the vestibular pathway rather than enhancing the multisensory aspects of compensation, asymmetry can be normalized within 1-2 sessions and show lasting effects.

Chemistry

ARL Spectral Fitting as an Application to Augment Spectral Data via Franck-Condon Lineshape Analysis and Color Analysis

William R. Roberts^1,2, Thomas N. Rohrabaugh¹, Ryan M. O'Donnell¹

¹DEVCOM Army Research Laboratory (ARL), ²Department of Chemistry, State University of New York at Buffalo

This protocol introduces Franck-Condon Lineshape Analyses (FCLSA) of emission spectra and serves as a tutorial for the use of ARL Spectral Fitting software. The open-source software provides an easy and intuitive way to perform advanced analysis of emission spectra including excited state energy calculations, CIE color coordinate determination, and FCLSA.

Biology

Realistic Membrane Modeling Using Complex Lipid Mixtures in Simulation Studies

Oluwatoyin Campbell¹, Van Le², Angela Aguirre¹, Viviana Monje-Galvan¹

¹Department of Chemical and Biological Engineering, State University of New York at Buffalo, ²Department of Mathematics, State University of New York at Buffalo

Membrane lipid diversity in structure and composition is an important contributor to cellular processes and can be a marker of disease. Molecular dynamics simulations allow us to study membranes and their interactions with biomolecules at atomistic resolution. Here, we provide a protocol to build, run, and analyze complex membrane systems.