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University of Murcia

2 ARTICLES PUBLISHED IN JoVE

Medicine

External Cephalic Version: Is it an Effective and Safe Procedure?

Javier Sánchez-Romero¹, Vanesa García-Soria², Fernando Araico-Rodríguez¹, Javier Herrera-Giménez¹, José E. Blanco-Carnero^1,3, Aníbal Nieto-Díaz^1,3,4, María Luisa Sánchez-Ferrer^1,3,4

¹Department of Obstetrics & Gynecology, "Virgen de la Arrixaca", University Clinical Hospital, ²Department of Gynecology, "Reina Sofía", University Clinical Hospital, ³Department of Obstetrics and Gynecology, Pediatrics and Surgery, University of Murcia, ⁴Institute for Biomedical Research of Murcia

This article shows how to perform the external cephalic version (ECV) by two experienced obstetricians in the obstetric operating room in the presence of an anesthesiologist and a midwife. The ECV is carried out with analgesia and tocolysis. Two attempts are made under ultrasonography control.

Biology

Autofluorescence Imaging to Evaluate Red Algae Physiology

Teresa Coronado-Parra¹, Mónica Roldán^2,3, Marina Aboal⁴

¹Microscopy Core Facility, Technological Research and Scientific Area (ACTI), University of Murcia, ²Confocal Microscopy and Cellular Imaging Unit, Genetic and Molecular Medicine Department, Pediatric Institute for Rare Diseases, Hospital Sant Joan de Déu, ³Research Institute Sant Joan de Déu, Esplugues de Llobregat, Spain 4Algology Laboratory, Biology Faculty, University of Murcia, ⁴Algology Laboratory, Biology Faculty, University of Murcia

The present protocol describes step-by-step autofluorescence imaging and evaluation of phycobiliprotein changes in red algae based on spectral analysis. This is a label-free and non-destructive method to evaluate cellular adaptation to extreme habitats, when only scarce material is available and cells grow slowly, or not at all, under laboratory conditions.