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Royal North Shore Hospital

3 ARTICLES PUBLISHED IN JoVE

Bioengineering

Layered Alginate Constructs: A Platform for Co-culture of Heterogeneous Cell Populations

Poonam Sharma¹, Julianne D. Twomey¹, Michelle Patkin¹, Adam H. Hsieh¹

¹Fischell Department of Bioengineering, University of Maryland

Engineering and analysis of load bearing tissues with heterogeneous cell populations are still a challenge. Here, we describe a method for creating bi-layered alginate hydrogel discs as a platform for co-culture of diverse cell populations within one construct.

Bioengineering

Transplantation of a 3D Bioprinted Patch in a Murine Model of Myocardial Infarction

Christopher D. Roche^1,2,3,4, Carmine Gentile^1,2,3

¹The University of Sydney, ²University of Technology Sydney (UTS), ³The Royal North Shore Hospital, ⁴University Hospital of Wales

This protocol aims to transplant a 3D bioprinted patch onto the epicardium of infarcted mice modeling heart failure. It includes details regarding anesthesia, the surgical chest opening, permanent ligation of the left anterior descending (LAD) coronary artery and application of a bioprinted patch onto the infarcted area of the heart.

Bioengineering

Cardiac Spheroids as in vitro Bioengineered Heart Tissues to Study Human Heart Pathophysiology

Poonam Sharma^1,2,3,4, Carmine Gentile^2,3,4

¹University of Newcastle, ²University of Sydney, ³Kolling Institute of Medical Research, Royal North Shore Hospital, ⁴University of Technology, Sydney

This protocol aims to fabricate 3D cardiac spheroids (CSs) by co-culturing cells in hanging drops. Collagen-embedded CSs are treated with doxorubicin (DOX, a cardiotoxic agent) at physiological concentrations to model heart failure. In vitro testing using DOX-treated CSs may be used to identify novel therapies for heart failure patients.