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Statens Serum Institut

4 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Ex Vivo Culture of Pharyngeal Arches to Study Heart and Muscle Progenitors and Their Niche

¹Division of Cardiology, Institute for Cell Engineering, Johns Hopkins University School of Medicine

Here, we present a protocol to culture pharyngeal arches to study the biology of heart and muscle progenitor cells and their microenvironment.

Genetics

Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach

Jakob Frimodt-Møller¹, Godefroid Charbon¹, Karen A. Krogfelt², Anders Løbner-Olesen¹

¹Department of Biology, Section for Functional Genomics and Center for Bacterial Stress Response and Persistence (BASP), University of Copenhagen, ²Department of Microbiology and Infection Control, Statens Serum Institut

Here, the power of a transposon-mediated random insertion of a non-coding DNA element was used to resolve its optimal chromosomal position.

Immunology and Infection

A Suction Blister Protocol to Study Human T-cell Recall Responses In Vivo

Line L. Holm^1,2,3, Milica Vukmanovic-Stejic⁴, Thomas Blauenfeldt¹, Thomas Benfield^2,3, Peter Andersen¹, Arne N. Akbar⁴, Morten Ruhwald¹

¹Department of Infectious Disease Immunology, Center for Vaccine Research, Statens Serum Institut, ²Department of Infectious Diseases, Hvidovre Hospital, ³Institute of Clinical Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, ⁴Division of Infection and Immunity, University College London

Here, we provide a demonstration of the suction blister cutaneous recall model. The model allows a simple access to study human in vivo adaptive immune responses, for instance in the context of vaccine development.

Immunology and Infection

Rapid, Safe, and Simple Manual Bedside Nucleic Acid Extraction for the Detection of Virus in Whole Blood Samples

Maiken W. Rosenstierne¹, Christopher E. Jensen¹, Anders Fomsgaard^1,2

¹Virus Research & Development Laboratory, Statens Serum Institut, ²Infectious Disease Research Unit, University of Southern Denmark

Here, we present a protocol for the rapid virus nucleic acid extraction from the virus-inactivated whole blood. The extraction is performed directly in the blood collection tubes and requires no equipment or electricity. The method is not dependent on laboratory facilities and can be used anywhere (e.g., in field hospitals).