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University of Regensburg

4 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Killer Artificial Antigen Presenting Cells (KaAPC) for Efficient In Vitro Depletion of Human Antigen-specific T Cells

Christian Schütz¹, Martin Fleck^2,3, Jonathan P. Schneck¹, Mathias Oelke¹

¹Department of Pathology, Institute of Cell Engineering, Johns Hopkins University, ²Department of Internal Medicine I, University of Regensburg, ³Department of Rheumatology, Asklepios Medical Center

Guidelines are presented for the generation of killer artificial antigen presenting cells, KaAPC, an efficient tool for in vitro depletion of human antigen-specific T cells and an alternative solution to cellular immunotherapy for the treatment of T cell-mediated autoimmune diseases in an antigen-specific fashion without compromising the remaining immune system.

Biology

The Mouse Isolated Perfused Kidney Technique

Jan Czogalla¹, Frank Schweda², Johannes Loffing¹

¹Institute of Anatomy, Swiss National Centre of Competence in Research Kidney, University of Zürich, ²Department of Physiology, University of Regensburg

The mouse isolated perfused kidney (MIPK) is a technique for keeping a mouse kidney under ex vivo conditions perfused and functional for 1 hr. The buffers and surgical technique are described in detail.

JoVE Core

Stepwise Dosing Protocol for Increased Throughput in Label-Free Impedance-Based GPCR Assays

Judith A. Stolwijk¹, Anne-Kathrin Mildner¹, Christian Kade¹, Michael Skiba¹, Guenther Bernhardt², Armin Buschauer², Harald Huebner³, Peter Gmeiner³, Joachim Wegener^1,4

¹Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, ²Institute of Pharmacy, University of Regensburg, ³Department of Chemistry and Pharmacy, Friedrich-Alexander University Erlangen-Nuernberg, ⁴Fraunhofer Research Institution for Microsystems and Solid State Technologies EMFT

This protocol demonstrates real-time recording of full dose-response relationships for agonist-induced GPCR activation from a single cell layer grown on a single microelectrode using label-free impedance measurements. The new dosing scheme significantly increases throughput without loss in time resolution.

Engineering

Evaluating Usability Aspects of a Mixed Reality Solution for Immersive Analytics in Industry 4.0 Scenarios

Burkhard Hoppenstedt¹, Thomas Probst², Manfred Reichert¹, Winfried Schlee³, Klaus Kammerer¹, Myra Spiliopoulou⁴, Johannes Schobel¹, Michael Winter¹, Anna Felnhofer⁵, Oswald D. Kothgassner⁶, Rüdiger Pryss⁷

¹Institute of Databases and Information Systems, Ulm University, ²Department for Psychotherapy and Biopsychosocial Health, Danube University Krems, ³Department of Psychiatry and Psychotherapy, University of Regensburg, ⁴Faculty of Computer Science, Otto von Guericke University Magdeburg, ⁵Department of Pediatrics and Adolescent Medicine, Medical University of Vienna, ⁶Department of Child and Adolescent Psychiatry, Medical University of Vienna, ⁷Institute of Clinical Epidemiology and Biometry, University of Würzburg

This protocol delineates the technical setting of a developed mixed reality application that is used for immersive analytics. Based on this, measures are presented, which were used in a study to gain insights into usability aspects of the developed technical solution.