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Harvard-MIT Division of Health Sciences and Technology

2 ARTICLES PUBLISHED IN JoVE

Bioengineering

Studying Cell Rolling Trajectories on Asymmetric Receptor Patterns

Chia-Hua Lee¹, Suman Bose², Krystyn J. Van Vliet¹, Jeffrey M. Karp³, Rohit Karnik²

¹Department of Materials Science and Engineering, MIT - Massachusetts Institute of Technology, ²Department of Mechanical Engineering, MIT - Massachusetts Institute of Technology, ³HST Center for Biomedical Engineering and Harvard Stem Cell Institute, Brigham and Women's Hospital and Harvard Medical School

We describe a protocol to observe and analyze cell rolling trajectories on asymmetric receptor-patterned substrates. The resulting data are useful for engineering of receptor-patterned substrates for label-free cell separation and analysis.

Bioengineering

Systematic Analysis of In Vitro Cell Rolling Using a Multi-well Plate Microfluidic System

Oren Levy^1,2,3,4,5, Priya Anandakumaran^1,2,3,4,5, Jessica Ngai^1,2,3,4,5, Rohit Karnik⁶, Jeffrey M. Karp^1,2,3,4,5

¹Division of Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, ²Center for Regenerative Therapeutics, Brigham and Women's Hospital, ³Harvard Medical School, Harvard University, ⁴Harvard Stem Cell Institute, Harvard University, ⁵Harvard-MIT Division of Health Sciences and Technology, ⁶Department of Mechanical Engineering, Massachusetts Institute of Technology

This study used a multi-well plate microfluidic system, significantly increasing throughput of cell rolling studies under physiologically relevant shear flow. Given the importance of cell rolling in the multi-step cell homing cascade and the importance of cell homing following systemic delivery of exogenous populations of cells in patients, this system offers potential as a screening platform to improve cell-based therapy.