Imaging of HIV-1 Envelope-induced Virological Synapse and Signaling on Synthetic Lipid BilayersKathleen C. Prins *1,2, Gaia Vasiliver-Shamis *2,3, Michael Cammer 1,2, David Depoil 1,2, Michael L. Dustin 2, Catarina E. Hioe 1,4
1Department of Pathology, New York University Langone School of Medicine, 2Program in Molecular Pathogenesis, Marty and Helen Kimmel Center for Biology and Medicine and Skirball Institute for Biomolecular Medicine, 3Laboratory of Molecular Immunogenetics, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, 4Veteran Affairs New York Harbor Healthcare System
This article describes a method to visualize formation of an HIV-1 envelope-induced virological synapse on glass supported planar bilayers by total internal reflection fluorescence (TIRF) microscopy. The method can also be combined with immunofluorescence staining to detect activation and redistribution of signaling molecules that occur during HIV-1 envelope-induced virological synapse formation.
