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University of Patras

3 ARTICLES PUBLISHED IN JoVE

Bioengineering

A GMP-Compliant Procedure for the Generation of Gene-Modified T cells

Nikolaos Savvopoulos¹, Klearchos Stampolitis¹, Georgios Alexandropoulos¹, Dionysia Kefala¹, Memnon Lysandrou¹, Vassiliki Zacharioudaki¹, Nikos Tsolakos², Alexandros Spyridonidis¹

¹Institute of Cell Therapy, Bone Marrow Transplantation Lab, Department of Medicine, University of Patras, ²Protatonce Ltd, Demokritos Science Park

This protocol outlines the process of transducing primary human T cells with a gene of interest, ensuring compatibility with Good Manufacturing Practice (GMP) standards.

Neuroscience

The "Brain Milking" Method for the Isolation of Neural Stem Cells and Oligodendrocyte Progenitor Cells from Live Rats

Dimitrios Dimitrakopoulos¹, Chrisitna Dimitriou¹, Freyja McClenahan², Robin J. M. Franklin^2,3, Ilias Kazanis¹

¹Laboratory of Developmental Biology, Department of Biology, University of Patras, ²Wellcome Trust-MRC Cambridge Stem Cell Institute, University of Cambridge, ³Altos Labs, Cambridge Institute of Science

A method for the isolation of neural stem cells and oligodendrocyte progenitor cells from the brains of live rats is presented here in experimental detail. It allows multiple collections of these cells from the same animals without compromising their well-being.

Biology

Murine Dermal Lymphatic Endothelial Cell Isolation

Racheal Grace Akwii^*1, Margarita Lamprou^*2, Maria Georgomanoli³, Andriana Plevriti², Antonia Marazioti⁴, Athanasia Mouzaki⁵, George Mattheolabakis⁶, Constantinos M. Mikelis^1,2

¹Department of Pharmaceutical Sciences, School of Pharmacy, Texas Tech University Health Sciences Center, ²Laboratory of Molecular Pharmacology, Department of Pharmacy, University of Patras, ³Division of Flow Cytometry and Division of MDx & Life Sciences, SB BioAnalytica, ⁴Basic Sciences Laboratory, Department of Physiotherapy, University of Peloponnese, ⁵Division of Hematology, Department of Internal Medicine, Medical School, University of Patras, ⁶School of Basic Pharmaceutical and Toxicological Sciences, College of Pharmacy, University of Louisiana at Monroe

This paper describes a method for magnetic bead-based isolation of murine endothelial cells from dermal lymphatic capillaries. The isolated lymphatic endothelial cells can be used for downstream in vitro experiments and protein expression analysis.