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LSU Health Sciences Center - New Orleans

3 ARTICLES PUBLISHED IN JoVE

Neuroscience

Isolation and Culture of Rat Embryonic Neural Cells: A Quick Protocol

Marco Pacifici^1,2, Francesca Peruzzi^1,2

¹LSU Health Sciences Center - New Orleans, ²Medical School and Stanley S. Scott Cancer Center

We describe a rapid methodology to isolate and culture hippocampal and cortical neurons from rodent embryos. This protocol allows us to perform experiments in which nearly pure neuronal cultures are required.

Medicine

Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR

Marco Pacifici¹, Serena Delbue², Ferdous Kadri¹, Francesca Peruzzi¹

¹Medical School and Stanley S. Scott Cancer Center, LSU Health Sciences Center, ²Department of Biomedical, Surgery and Dental Sciences, University of Milan

We describe a protocol of real time PCR to profile microRNAs in the cerebrospinal fluid (CSF). With the exception of RNA extraction protocols, the procedure can be extended to RNA extracted from other body fluids, cultured cells, or tissue specimens.

Immunology and Infection

Isolation, Transfection, and Culture of Primary Human Monocytes

Karlie Plaisance-Bonstaff^1,2, Celeste Faia², Dorota Wyczechowska¹, Duane Jeansonne¹, Cecilia Vittori^1,3, Francesca Peruzzi^1,2

¹Department of Medicine, Louisiana State University Health Sciences Center, ²Department of Microbiology, Immunology, and Parasitology, Louisiana State University Health Sciences Center, ³Department of Biomedical and Clinical Sciences L. Sacco, University of Milan

Presented here is an optimized protocol for isolating, culturing, transfecting, and differentiating human primary monocytes from HIV-infected individuals and healthy controls.