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Guizhou Normal University

3 ARTICLES PUBLISHED IN JoVE

Cancer Research

An Integrated Platform for Genome-wide Mapping of Chromatin States Using High-throughput ChIP-sequencing in Tumor Tissues

Christopher Terranova¹, Ming Tang¹, Elias Orouji¹, Mayinuer Maitituoheti¹, Ayush Raman¹, Samirkumar Amin², Zhiyi Liu¹, Kunal Rai¹

¹Department of Genomic Medicine, University of Texas MD Anderson Cancer Center, ²The Jackson Laboratory for Genomic Medicine

Here, we describe an optimized high-throughput ChIP-sequencing protocol and computational analyses pipeline for the determination of genome-wide chromatin state patterns from frozen tumor tissues and cell lines.

Genetics

Inducing Hairy Roots by Agrobacterium rhizogenes-Mediated Transformation in Tartary Buckwheat (Fagopyrum tataricum)

Yaolei Mi^1,2, Zhihui Zhu³, Guangtao Qian³, Yu Li⁴, Xiangxiao Meng¹, Jianping Xue³, Qingfu Chen⁵, Wei Sun¹, Yuhua Shi^1,2

¹Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, ²Artemisinin Research Center, China Academy of Chinese Medical Sciences, ³College of Life Science, Huaibei Normal University, ⁴Economic Crop Research Institute Sichuan Academy of Agriculture Sciences, ⁵Research Center of Buckwheat Industry Technology, Guizhou Normal University

We describe a method of inducing hairy roots by Agrobacterium rhizogenes-mediated transformation in Tartary buckwheat (Fagopyrum tataricum). This can be used to investigate gene functions and production of secondary metabolites in Tartary buckwheat, be adopted for any genetic transformation, or used for other medicinal plants after improvement.

Biology

Divergence of Root Microbiota in Different Habitats based on Weighted Correlation Networks

Jing Tang^*1,2, Xinzhu He^*1, Ming Tang¹, Xiaorong Xu², Ximin Zhang¹

¹Key Laboratory of State Forestry Administration on Biodiversity Conservation in Karst Mountainous Areas of Southwestern China, School of Life Sciences, Guizhou Normal University, ²State Key Laboratory of Plant Physiology and Development in Guizhou Province, Guizhou Normal University

Network analysis was applied to evaluate the association of various ecological microbial communities, such as soil, water and rhizosphere. Presented here is a protocol on how to use the WGCNA algorithm to analyze different co-occurrence networks that may occur in the microbial communities due to different ecological environments.