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Vanderbilt University Medical School

3 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Staphylococcus aureus Growth using Human Hemoglobin as an Iron Source

Gleb Pishchany¹, Kathryn P. Haley¹, Eric P. Skaar¹

¹Department of Pathology, Microbiology and Immunology, Vanderbilt University Medical School

Here we describe a growth assay for Staphylococcus aureus using hemoglobin as the sole source of available nutrient iron. This assay establishes the role of bacterial factors involved in hemoglobin-derived iron acquisition.

Developmental Biology

Effective Isolation of Functional Islets from Neonatal Mouse Pancreas

Chen Huang¹, Guoqiang Gu¹

¹Department of Cell and Development Biology, Vanderbilt University Medical School

We describe a protocol herein for isolating intact islets from neonatal mice. Pancreata were partially digested with collagenase, followed by washing and hand picking. 20 - 80 islet clusters can be obtained per pancreas from newly born mice, which are suitable for several islet studies.

Biochemistry

Expression, Purification, and Antimicrobial Activity of S100A12

Emmanuel Jackson¹, Saffron Little¹, Dana S. Franklin¹, Jennifer A. Gaddy^2,3, Steven M. Damo^1,4

¹Department of Life and Physical Sciences, Fisk University, ²Tennessee Valley Healthcare Systems, U. S. Dept. of Veterans Affairs, ³Department of Medicine - Division of Infectious Diseases, Vanderbilt University Medical School, ⁴Department of Biochemistry, Vanderbilt University

Here, we present a method to express and purify S100A12 (calgranulin C). We describe a protocol to measure its antimicrobial activity against the human pathogen H. pylori.