The overall goal of this procedure is to isolate intact islets of Langerhans from the neonatal mouse pancreas. This method can help answer the key questions in adding development and function. Such as insulin excretion, gene expression, para cell
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We describe a protocol herein for isolating intact islets from neonatal mice. Pancreata were partially digested with collagenase, followed by washing and hand picking. 20 - 80 islet clusters can be obtained per pancreas from newly born mice, which are suitable for several islet studies.