Silencing the Spark: CRISPR/Cas9 Genome Editing in Weakly Electric FishSavvas J. Constantinou 1, Linh Nguyen 2, Frank Kirschbaum 2, Vielka L. Salazar 3, Jason R. Gallant 1
1Department of Integrative Biology, Michigan State University, 2Faculty of Life Sciences, Unit of Biology and Ecology of Fishes, Humboldt University, 3Department of Biology, Cape Breton University
Here, a protocol is presented to produce and rear CRISPR/Cas9 genome knockout electric fish. Outlined in detail are the required molecular biology, breeding, and husbandry requirements for both a gymnotiform and a mormyrid, and injection techniques to produce Cas9-induced indel F0 larvae.
