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Beijing Institute of Basic Medical Sciences

1 ARTICLES PUBLISHED IN JoVE

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Neuroscience

An Invasive Method for the Activation of the Mouse Dentate Gyrus by High-frequency Stimulation
Zhe Zhao 1, Haitao Wu 1,2
1Department of Neurobiology, Beijing Institute of Basic Medical Sciences, 2Key Laboratory of Neuroregeneration, Co-innovation Center of Neuroregeneration, Nantong University

This protocol shows how to set up a reliable HFS method in mice. Neurons throughout the hippocampal dentate gyrus are electrically stimulated by HFS directly and indirectly in vivo. Neuronal activity and molecular signaling are examined by c-fos and Notch1 immunofluorescent staining, respectively; neurogenesis is quantified by bromodeoxyuridine labeling assay.

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