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Dankook University

3 ARTICLES PUBLISHED IN JoVE

Medicine

Time-lapse Imaging of Primary Preneoplastic Mammary Epithelial Cells Derived from Genetically Engineered Mouse Models of Breast Cancer

Rebecca E. Nakles¹, Sarah L. Millman¹, M. Carla Cabrera¹, Peter Johnson^1,2, Susette Mueller^1,2, Philipp S. Hoppe³, Timm Schroeder³, Priscilla A. Furth^1,2,4,5

¹Department of Oncology, Georgetown University, ²Lombardi Comprehensive Cancer Center, Georgetown University, ³Stem Cell Dynamics, Helmholtz Zentrum München - German Research Center for Environmental Health, ⁴Department of Medicine, Georgetown University, ⁵Department of Nanobiomedical Science and WCU Research Center of Nanobiomedical Science, Dankook University

Time-lapse imaging is used to assess behavior of primary preneoplastic mammary epithelial cells derived from genetically engineered mouse models of breast cancer risk to determine if there are correlations between specific behavioral parameters and distinct genetic lesions.

Chemistry

Quantitative and Qualitative Examination of Particle-particle Interactions Using Colloidal Probe Nanoscopy

Dexter D'Sa¹, Hak-Kim Chan¹, Hae-Won Kim², Wojciech Chrzanowski^1,2

¹Faculty of Pharmacy, University of Sydney, ²Department of Nanobiomedical Science & BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University

Colloidal probe nanoscopy can be used within a variety of fields to gain insight into the physical stability and coagulation kinetics of colloidal systems and aid in drug discovery and formulation sciences using biological systems. The method described within provides a quantitative and qualitative means to study such systems.

Developmental Biology

Loss- and Gain-of-function Approach to Investigate Early Cell Fate Determinants in Preimplantation Mouse Embryos

Jae H. Lee^1,2, Yong II Cho⁵, Sung S. Choi⁴, Hae-Won Kim^1,2, Churl K. Min³, Sang J. Lee⁵

¹Department of Nanobiomedical Sciences and BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, ²Insititute of Tissue Regeneration Engineering, Dankook University, ³Department of Biological Sciences, Ajou University, ⁴Department of Pharmacy, Sahmyook University, ⁵Department of Animal Biotechnology, Sahmyook University

The goal of this protocol is to describe a loss- and gain-of function method that is applicable to identify neogenin as a stage-specific receptor that leads to trophectoderm and inner cell mass differentiation in preimplantation mouse embryos.