Retrieval and Culturing of 2-Pronuclear (2-PN) Embryos
3:46
Microinjecting 2-PN Embryos
6:29
Results: Neogenin Expression Manipulations
7:59
Conclusion
Transcrição
The overall goal of this protocol is to use loss-and gain-of-function manipulations to identify stage-specific molecules that control inner cell mass differentiation of mouse embryos during pre-implantation. This method can help answer the key que
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The goal of this protocol is to describe a loss- and gain-of function method that is applicable to identify neogenin as a stage-specific receptor that leads to trophectoderm and inner cell mass differentiation in preimplantation mouse embryos.