A Non-Degradative Extraction Method for Molecular Structure Characterization of Bacterial Glycogen ParticlesJun-Jiao Wang *1, Meng-Meng Wang *2,3, You-Wei He 4, Zhang-Wen Ma 2,3, Zi-Yi Wang 5,6,7, Rui Qiao 2,3, Xin-Le Tan 5,6, Qing-Hua Liu 8, Liang Wang 1,2
1Department of Bioinformatics, School of Medical Informatics and Engineering, Xuzhou Medical University, 2Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, School of Pharmacy, Xuzhou Medical University, 3Department of Pharmaceutical Analysis, School of Pharmacy, Xuzhou Medical University, 4Department of Biological Sciences, School of Life Sciences, Xuzhou Medical University, 5School of Chemistry and Molecular Biosciences, The University of Queensland, 6Centre for Nutrition and Food Sciences, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, 7Joint International Research Laboratory of Agriculture and Agri-Product Safety, College of Agriculture, Yangzhou University, 8State Key Laboratory of Quality Research in Chinese Medicines, Macau University of Science and Technology
Bacterial glycogen structure is greatly impacted by extraction methods which may result in molecular degradation and/or biased sampling. It is essential to develop methods to minimize these problems. Here, four extraction methods have been compared using size distribution and chain length distribution as key criteria for minimizing extraction artifacts.
