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Instituto de Investigaciones Biológicas Clemente Estable

2 ARTICLES PUBLISHED IN JoVE

Biology

Simple and Efficient Technique for the Preparation of Testicular Cell Suspensions

Rosana Rodríguez-Casuriaga¹, Gustavo A. Folle², Federico Santiñaque², Beatriz López-Carro², Adriana Geisinger^1,3

¹Departamento de Biología Molecular, Instituto de Investigaciones Biológicas Clemente Estable (IIBCE), ²Servicio de Citometría de Flujo y Clasificación Celular (SECIF), Instituto de Investigaciones Biológicas Clemente Estable, ³Sección Bioquímica, Facultad de Ciencias, Universidad de la República

A novel protocol for the mechanical preparation of testicular cell suspensions from rodent material, avoiding enzymes and detergents, is described. The method is very simple, fast, reproducible, and renders good quality cell suspensions, which are suitable for flow sorting and RNA extraction.

Developmental Biology

Polysome Purification from Soybean Symbiotic Nodules

María Martha Sainz¹, Carla Valeria Filippi¹, Guillermo Eastman^2,3, Mariana Sotelo-Silveira¹, C. Mauro Martinez¹, Omar Borsani¹, José Sotelo-Silveira^2,4

¹Laboratorio de Bioquímica, Departamento de Biología Vegetal, Facultad de Agronomía, Universidad de la República, ²Departamento de Genómica, Instituto de Investigaciones Biológicas Clemente Estable, ³Department of Biology, University of Virginia, ⁴Departamento de Biología Celular y Molecular, Facultad de Ciencias, Universidad de la República

This protocol describes a method for eukaryotic polysome purification from intact soybean nodules. After sequencing, standard pipelines for gene expression analysis can be used to identify differentially expressed genes at the transcriptome and translatome levels.