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Chengdu University

1 ARTICLES PUBLISHED IN JoVE

Biology

Noninvasive Intratracheal Lipopolysaccharide Instillation in Mice

Peng Yu^*1, Bo Lin^*2, Jingyang Li¹, Yanqing Luo¹, Di Zhang¹, Jiayi Sun¹, Xianli Meng¹, Yingfan Hu³, Li Xiang¹

¹State Key Laboratory of Southwestern Chinese Medicine Resources, College of Pharmacy, Innovative Institute of Chinese Medicine and Pharmacy, Chengdu University of Traditional Chinese Medicine, ²Department of Pharmacy, The Second Affiliated Hospital of Hainan Medical University, ³The School of Preclinical Medicine, Chengdu University

Here, we propose a protocol for intratracheal lipopolysaccharide (LPS) delivery via noninvasive oropharyngeal endotracheal intubation. This method minimizes the trauma of the surgical procedure for the animal and accurately delivers LPS to the trachea and then to the lungs.