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Indiana University School of Medicine - Terre Haute

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Histological Quantification to Determine Lung Fungal Burden in Experimental Aspergillosis

Dylan J. Stolz¹, Ethan M. Sands¹, Nansalmaa Amarsaikhan¹, Angar Tsoggerel¹, Steven P. Templeton¹

¹Department of Microbiology and Immunology, Indiana University School of Medicine - Terre Haute

Here we describe a protocol to determine pulmonary fungal burden in mice with invasive aspergillosis by quantification of Gomori's modified methanamine silver staining in histological sections. Use of this method resulted in comparable results with less animals compared to assessment of fungal burden by quantitative PCR of lung fungal DNA.