Histological Quantification to Determine Lung Fungal Burden in Experimental AspergillosisDylan J. Stolz 1, Ethan M. Sands 1, Nansalmaa Amarsaikhan 1, Angar Tsoggerel 1, Steven P. Templeton 1
1Department of Microbiology and Immunology, Indiana University School of Medicine - Terre Haute
Here we describe a protocol to determine pulmonary fungal burden in mice with invasive aspergillosis by quantification of Gomori's modified methanamine silver staining in histological sections. Use of this method resulted in comparable results with less animals compared to assessment of fungal burden by quantitative PCR of lung fungal DNA.
