Reproducible dsRNA Microinjection and Oviposition Bioassay in Mosquitoes and House FliesNeil D. Sanscrainte 1, Christy M. Waits 2,3, Christopher J. Geden 1, Alden S. Estep 1,2, James J. Becnel 1
1USDA/ARS Center for Medical, Agricultural, and Veterinary Entomology, 2CMAVE Detachment, Navy Entomology Center of Excellence, 3Lovelace Respiratory Research Institute
This protocol describes a microinjection methodology that we have standardized and used for several years to deliver specific quantities of nucleic acids directly to the hemolymph of mosquitoes and house flies. This protocol results in minimal injection mortality and allows dose correlated measurements of fecundity.
