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Navy Entomology Center of Excellence

1 ARTICLES PUBLISHED IN JoVE

Environment

Reproducible dsRNA Microinjection and Oviposition Bioassay in Mosquitoes and House Flies

Neil D. Sanscrainte¹, Christy M. Waits^2,3, Christopher J. Geden¹, Alden S. Estep^1,2, James J. Becnel¹

¹USDA/ARS Center for Medical, Agricultural, and Veterinary Entomology, ²CMAVE Detachment, Navy Entomology Center of Excellence, ³Lovelace Respiratory Research Institute

This protocol describes a microinjection methodology that we have standardized and used for several years to deliver specific quantities of nucleic acids directly to the hemolymph of mosquitoes and house flies. This protocol results in minimal injection mortality and allows dose correlated measurements of fecundity.