Real-time Cytotoxicity Assays in Human Whole BloodChing-Wen Hsiao *1, Yen-Ting Lo *1, Hong Liu 2, Sonny C. Hsiao 1
1Research and Development, Adheren, Inc, 2Research and Development, Eureka Therapeutics
The whole blood cytotoxicity assay (WCA) is a cytotoxicity assay developed by incorporating high-throughput cell positioning technology with fluorescence microscopy and automated image processing. Here, we describe how lymphoma cells treated with an anti-CD20 antibody can be analyzed real-time in human whole blood to provide quantitative cellular cytotoxicity analysis.
