Apoplast-Extraction Based Method to Improve the Purity of Plant Produced Recombinant Protein Huiying Ji 1,2, Tianhao Zhang 2, Han Li 2,3, Chunyu Wang 1,2, Rongxiang Fang 2, Lili Zhang 1,2,4, Yan Huo 2
1Shandong Academy of Medical Sciences, Shandong First Medical University, 2State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, 3College of Veterinary Medicine, Shanxi Agricultural University, 4Beijing Life Science Academy
The purification of recombinant proteins from plant systems is usually challenged by plant proteins. This work provides a method to effectively extract and purify a secreted recombinant protein from the apoplast of Nicotiana benthamiana.
