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Institute of Biosciences and Technology - Texas A&M Health Science Center

4 ARTICLES PUBLISHED IN JoVE

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Biology

Method for Culture of Early Chick Embryos ex vivo (New Culture)
Delphine Psychoyos 1, Richard Finnell 2
1Center for Environmental and Genetic Medicine, Institute of Biosciences and Technology - Texas A&M Health Science Center , 2Center for Environmental and Genetic Medicine, Texas A&M University (TAMU)

This video demonstrates New culture, a method by which chick embryos are cultured outside the egg for up to 24 hr. This method enables one to study early development (primitive streak to 14 som.), a period corresponding to E7-9 in mouse. Applications of this technique include electroporation, in situ hybridization and immunohistochemistry.

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Biology

Double Whole Mount in situ Hybridization of Early Chick Embryos
Delphine Psychoyos 1, Richard Finnell 2
1Center for Environmental and Genetic Medicine, Institute of Biosciences and Technology - Texas A&M Health Science Center , 2Center for Environmental and Genetic Medicine, Texas A&M University (TAMU)

This video demonstrates 2-color whole mount in situ hybridization, a method by which the spatial and temporal expression pattern of 2 different genes can be visualized in young chick embryos. This method was originally introduced by David Wilkinson, Domingos Henrique, Phil Ingham and David Ish -Horowicz.

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Biology

Method for Whole Mount Antibody Staining in Chick
Delphine Psychoyos 1, Richard Finnell 1
1Institute of Biosciences and Technology, Center for Environmental and Genetic Medicine, Texas A&M University (TAMU)

This video demonstrates whole mount immunohistochemistry, a method by which the spatial and temporal expression pattern of an antigen can be visualized in young chick embryos. This method was originally introduced by Jane Dodd and Tom Jessell.

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Biology

Assay for Neural Induction in the Chick Embryo
Delphine Psychoyos 1, Richard Finnell 1
1Institute of Biosciences and Technology, Center for Environmental and Genetic Medicine, Texas A&M University (TAMU)

Neural induction is the first step in the formation of the brain. It is a mechanism by which Hensen's node (organizer), instructs adjacent tissue to adopt a neural fate, i.e. to give rise to the nervous system. This video demonstrates an assay for neural induction in chick embryo.

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