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Hubrecht Institute-KNAW and University Medical Center Utrecht

2 ARTICLES PUBLISHED IN JoVE

Biology

Dendra2 Photoswitching through the Mammary Imaging Window

Bojana Gligorijevic^1,2, Dmitriy Kedrin¹, Jeffrey E Segall¹, John Condeelis^1,2, Jacco van Rheenen^1,2,3

¹Department of Anatomy and Structural Biology, Albert Einstein College of Medicine - Yeshiva University, ²Gruss Lipper Biophotonics Center, Albert Einstein College of Medicine - Yeshiva University, ³Hubrecht Institute-KNAW and University Medical Center Utrecht

Intravital photoswitching and tracking of Dendra2-labeled tumor cells through the Mammary Imaging Window is a technique which allows us to image the metastatic behavior of tumor cells in chosen tumor microenvironments over a timescale of days.

Biology

Longitudinal Intravital Microscopy Using a Mammary Imaging Window with Replaceable Lid

Larissa Mourao^1,2, Marta Ciwinska^1,2, Jacco van Rheenen³, Colinda L. G. J. Scheele^1,2

¹Center for Cancer Biology, VIB (BE), ²Department of Oncology, KU Leuven (BE), ³Netherlands Cancer Institute, Department of Molecular Pathology, Oncode Institute (NL)

This protocol describes a novel mammary imaging window with a replaceable lid (R.MIW). Intravital microscopy after implantation of the R.MIW allows for longitudinal and multi-day imaging of the healthy and diseased mammary gland with a cellular resolution during the different developmental stages.