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Hadassah Medical School - Hebrew University

1 ARTICLES PUBLISHED IN JoVE

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Biology

Single Cell Transfection in Chick Embryos
Raz Ben-Yair 1, Chaya Kalcheim 1
1Department of Medical Neurobiology, Hadassah Medical School - Hebrew University

Using fine tip micropipettes we inject plasmid DNA into subdomains of chicken somites or neural tubes. The concentration of the plasmid is adjusted to generate single transfected cells. We then allow the cells to develop into clonal populations.

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