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University of Maryland School of Medicine and the Marlene and Stewart Greenebaum Comprehensive Cancer Center

5 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

Artificial Antigen Presenting Cell (aAPC) Mediated Activation and Expansion of Natural Killer T Cells
James E. East *1, Wenji Sun *1, Tonya J. Webb 1
1Department of Microbiology and Immunology, University of Maryland

Here we describe a method for activating and expanding human NKT cells from bulk T cell populations using artificial antigen presenting cells (aAPC). The use of CD1d-based aAPC provides a standardized method for generating high numbers of functional NKT cells.

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Advanced Biology

ELISPOT Assay: Detection of IFN-γ Secreting Splenocytes
Tonya J. Webb 1
1Department of Microbiology and Immunology, University of Maryland School of Medicine and the Marlene and Stewart Greenebaum Comprehensive Cancer Center

ELISPOT Assay: Detection of IFN-γ Secreting Splenocytes

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Advanced Biology

Immunohistochemistry and Immunocytochemistry: Tissue Imaging via Light Microscopy
Michael S. Lee 1, Tonya J. Webb 1
1Department of Microbiology and Immunology, University of Maryland School of Medicine and the Marlene and Stewart Greenebaum Comprehensive Cancer Center

Immunohistochemistry and Immunocytochemistry: Tissue Imaging via Light Microscopy

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Advanced Biology

Confocal Fluorescence Microscopy: A Technique to Determine the Localization of Proteins in Mouse Fibroblasts
Dominique R. Bollino 1, Eric A. Legenzov 2, Tonya J. Webb 1
1Department of Microbiology and Immunology, University of Maryland School of Medicine and the Marlene and Stewart Greenebaum Comprehensive Cancer Center, 2Center for Biomedical Engineering and Technology, University of Maryland School of Medicine

Confocal Fluorescence Microscopy: A Technique to Determine the Localization of Proteins in Mouse Fibroblasts

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Advanced Biology

Immunoprecipitation-Based Techniques: Purification of Endogenous Proteins Using Agarose Beads
Susannah C. Shissler 1, Tonya J. Webb 1
1Department of Microbiology and Immunology, University of Maryland

Immunoprecipitation-Based Techniques: Purification of Endogenous Proteins Using Agarose Beads

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