Here we describe a method for activating and expanding human NKT cells from bulk T cell populations using artificial antigen presenting cells (aAPC). The use of CD1d-based aAPC provides a standardized method for generating high numbers of functional NKT cells.
ELISPOT Assay: Detection of IFN-γ Secreting Splenocytes
Immunohistochemistry and Immunocytochemistry: Tissue Imaging via Light Microscopy
Confocal Fluorescence Microscopy: A Technique to Determine the Localization of Proteins in Mouse Fibroblasts
Immunoprecipitation-Based Techniques: Purification of Endogenous Proteins Using Agarose Beads
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